| Molecular imprinting technology?MIT?is a novel chemical analysis method which prepares polymer with specialized recognition for the template molecule.The molecular imprinted polymer has some merits of preordainment,higher identifiability,the stability of resistance to the bad environment,reusablity and so on.Compared with other method of molecularly imprinted polymer?MIP?film preparation,electropolymerization has outstanding properties,for instance strong adhesion to the electrode surface,easy preparation,low cost,easy control of the film thickness and high reproducibility.The molecularly imprinted sensor was made by electropolymerization which become a research focus.Three electrochemical sensors are prepared by using cyclic voltammetry?CV?base on molecularly imprinted technology.The performances of the sensors were investigated by square wave voltammetry?SWV?.Furthermore,the MIP sensor was applied to the detection of pharmaceutical drug.Summary of the main content is as follows:?1?A molecularly imprinted polymer?MIP?film for the determination of ciprofloxacin?CPX?was electropolymerized onto a pencil graphite electrode by using pyrrole?Py?and o-phenylenediamine?o-PD?as functional monomers,ciprofloxacin as template molecule.Under the optimum experimental conditions,the results prove that the difference of SWV peak current showed a linear dependence on the logarithm of ciprofloxacin concentration in the ranges of 1.0×10-3-1.6×10-9mol/L?correlation coefficient of 0.9981?with the detection limit?S/N=3?of 7.58×10-11 mol/L.This sensor displayed good selective and reproducibility binding sites for ciprofloxacin.The prepared sensor was successfully applied to the determination of ciprofloxacin in practical samples.?2?Based on electropolymerized methods,an electrochemical molecularly imprinted sensor for enrofloxacin?EF?specific recognition was prepared on a pencil graphite electrode using enrofloxacin as a template,pyrrole?Py?and o-phenylenediamine?o-PD?as functional monomers.The performance of the electrochemical sensor was characterized by square wave voltammetry?SWV?.The experimental results proved that the sensor has good selectivity and high sensitivity to enrofloxacin molecular.The linear range is from 1.0×10-4to 1×10-9 mol/L with the detection limit of 6.57×10-13 mol/L?S/N=3?.The sensor has been successfully put into use the determination of enrofloxacin in compound enrofloxacin Tablets.?3?An electrochemical molecularly imprinted sensor for norfloxacin?NRF?was studied using molecular imprinting technology,pyrrole?Py?and o-phenylenediamine?o-PD?as functional monomers on the surface of pencil graphite electrode through electrochemical polymerization,the electrochemical behavior of the molecularly imprinted polymer sensor characterized by square wave voltammetry?SWV?.Under the optimal experimental conditions,the results indicated that the difference of peak current is a linear function of the negative logarithm of norfloxacin concentration in the range from 1.0×10-3-1.0×10-9mol/L?R=0.9992?with the detection limit of 3.69×10-11 mol/L.The developed sensor has a good selectivity to norfloxacin,and the sensor was applied to the determination of content of norfloxacin drugs with satisfactory results. |
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