Study Of Rapid Detection Of Dinp In Samples By Molecular Imprinted Sensor

It is Cloud agent incidents in 2011 Taiwan and the mainland drunkard wine plasticizer event in 2012 that once again raise worries about food safety.So detecting the content of plasticizer in food accurately and rapidly has become a hot topic in the research of the current analysis inspection field.As an important type of plasticizer,Phthalate different nonyl ester(DINP)is great toxic,carcinogenic,which could increase the incidence of reproductive malformation,and can be induced by change of sexual differentiation.At present,the method for determination of the plasticizer is mainly GC(GC-MS),high performance liquid chromatography(HPLC),gas chromatography(GC)and liquid mass combination method(LC-MS/MS).By these methods,there are many disadvantages,such as complex sample pretreatment,high operation cost,not strong method specificity,shortcomings long testing time and so on.Therefore,it has a very important significance of practical application to develop a simple preparation process,low cost,high sensitivity and fast detection method.DINP molecularly imprinted electrochemical sensor construction mainly includes DINP the preparation of molecularly imprinted polymer and construction of DINP electrochemical sensors.Firstly,molecular imprinting technology is used in thermal polymerization to prepare molecularly imprinted polymer particles(MIPs).By scanning electron microscope to observe the morphology of MIPs characteristics,and analyzing the principle of combining MIPs and DINP.Secondly,optimize the experimental conditions to get the best MIPs.Then,blend the optimization of MIPs and agarose solution and fix on the glassy carbon electrode to prepare DINP molecularly imprinted sensor sensitive film.At last,detect sensor electrical signals by electrochemical cyclic voltammetry(CV)method to realize the practical application of sensor.Molecularly imprinted electrochemical sensor had been structured to detect DINP rapidly,which has been used well to detect the actual samples.In wine samples containing DINP,the sensors can be combined with DINP molecular specifically and produce electrical signals.Meanwhile,in potassium ferricyanide solution,different concentrations of DINP make current intensity change differently.Through electrochemical sensor to detect electrical signal,for further analyzing the sensor's sensitivity and selectivity.At the same time,in this paper,the DINP molecularly imprinted electrochemical sensor sensitivity has been researched.The study find that when the concentration of DINP is 40?mol/L,the sensor current changes max which means the sensitivity of the sensor is strongest.While the electrochemical response signal of phthalic acid butyl benzyl ester(BBP),dimethyl phthalate(DMP),phthalate(2-ethylhexyl)ester(DEHP)is weak,that the sensor is not sensitive to the three analogues,so it has specific recognition ability for DINP.Sensor detection range for DINP is 5×10-8?1×10-6 mol/L.Detection limit for DINP is 2.7×10-8 mol/L.The sensitivity is very well.By studying the DINP molecularly imprinted electrochemical sensor detection mechanism,we find that the specificity of the sensor for DINP molecular adsorption mainly through the combination of physical function and chemical action.DINP molecule combine with the hole structure on the molecularly imprinted polymer.Then through the hydrogen bonding interaction between DINP and functional monomer unifies in together more stable,which implement specific recognition ability.In this paper,we prepare DINP molecularly imprinted electrochemical sensor creatively with the method of molecular imprinting technology and electrochemistry.Through the optimization of experimental conditions,we detect the content of DINP in the samples accurately and rapidly.There are many advantages in this method,such as strong specificity,low cost,simple operation,short test time,which is expected to provide a rapid,accurate and sensitive detection method for food safety supervision.The method of preparation of MIPs has strong ability to adsorpt DINP,for wine and food production in the process of adsorption removal of plasticizing agent has ideal application prospect.