Study On The Analytical Method Of Trans Fatty Acids In Infant And Young Children And Its Level Investigation

Trans fatty acids(Trans-fatty acid, referred to as TFA) refers to a molecuLe containing one or more trans(trans) non-conjugated double bonds of unsaturated fatty acids in general, the presence of a small amount of trans fatty acids in infant food, it may be a major source of ruminant meat and dairy products, hydrogenated vegetable oil and vegetable oil refining deodorizing channels.Studies have shown that Trans fatty acids on the human body may have the following hazards: essential fatty acids interfere with the metabolism lead to cardiovascuLar disease, resuLting in a woman infertile, block fetal nervous system and children, affecting the growth and development of infants and young children.In this paper, infant formuLa milk powder and grain flour as the main object of study, to explore the optimum extraction of fatty acids in infant food in trans fatty acids to optimize muLti-component analysis of the conditions established on the basis of infant food by gas chromatography- mass spectrometry conduct research associated with analytical methods, and Jiangxi infant cereal powder content level.This paper main work and conclusions are as follows:1?To study the optimum infant foods in fat extracted. For infant food characteristics, using different methods comparison, selection and optimization of conditions, statistical analysis, the best way to establish the infant food fat extraction: the enzymatic- hydrolysis of ammonia- ethanol- petroleum ether extraction method of fat. Infant milk powder fat extraction mining a- amylase 20% of the amount of sample digestion 30 min, then hydrolysis 10 min, petroleum ether extraction using 50 mL best, this method on time from the traditional 140 min to 40 min, time efficiency 350% increase, fat extraction rate increased by 9.8%, reagent consumption savings of 50%; infant cereal flour used in aamylase 10% of the amount of sample digestion 60 min, 30 min and then subjected to hydrolysis using 50 mL of petroleum ether extract best this method increased by about 155% time-efficient than traditional methods, fat extraction rate increased by 22.61%, save 50% reagent. Fat extraction method of the newly established milk fat extraction rate reached 80.30 percent, the cereal flour in fat extraction rate of 81.74%.2?By gas chromatography- mass spectrometry for infant food mu Lti-component determination of Trans fatty acids.Explore infant food in trans fatty acids, potassium hydroxide- methanol and methylated derivatives optimal conditions, with 200 uL concentration 1moL/L potassium hydroxide- methanol refrigerated environment at-9 ? 1h, derived optimal effect.Compare HP-88 quartz capillary columns of different lengths, different temperature program for the separation of Trans fatty acid methyl ester, using mass spectrometry technique for component identification. In Agilent HP-88(100m × 0.25 mm × 0.20um) for the column inlet temperature of 240?, the injection volume 1uL, split ratio 10:1, the detector temperature 240?, temperature program 125?(0.5min) —10?/min —145?(0min) —5?/min—180?(15min) —5?/ min—240?(15min), in this condition analysis, effective separation of trans fatty acids in a sample comprising isomers. Isolation and separation of isomers of different kinds of thorough separation reached R>1.5(C18: 1-6t and C18: 1-9t excluded), the instrument sensitivity 0.01mg/L, this paper developed temperament chromatography method can be effective separation and identification of the sample muLti-component Trans fatty acids.3?Jiangxi Province investigated infant rice Trans fatty acids, using the internal standard method to analyze the relative content of each component. Found that trans fatty acids in infant foods prevalent, mainly C14: 1-9t, C16: 1-9t, C 18: 1-9t or C 18: 1-6t, C 18: 2-9t, 12 t, C19 : 1-7t structure and all-trans-linolenic acid C18: 3-9t, 12 t, 15 t and other components are present, the overall levels of cereal flour recipe is low, the affected individual ingredients, content is relatively high. Cereal powder. Comparison of different infant formuLa ingredients rice flour, rice flour infant nutrition found in Trans fatty acids mainly from ingredients of milk, protein powder and composite food nutrients.