Studied On Chloride Ion,heavy Metal And There Composite Stress Of Physiological Index And Enrichment Potential Of Myriophyllum Verticillatum L.

The Foxtail algal(Myriophyllum verticillatum L.)was studied as a experiment material which was treating with chloride ions,heavy metals and their combined stress,and the features of stress physiological and heavy metal accumulation were tested.The experiment results were as follows.1.Effects of different concentrations of chloride ion on the growth and physiological index of foxtail algae.Plants were treated by CI-in 28d,the growth and physiological indicators were measured that the weight of underground part was the lowest which was treated by 3g/L Cl-,difference significantly.Foxtail algal tolerance index was the highest treated by 2 g/L.The foxtail algae relative growth aboveground was the lowest that was treated by 5g/L CI-,difference significantly.The chlorophyll content to a minimum and the highest degree of cell membrane lipid damage which was treated by 5g/L CI-.SOD activities was the lowest treating with 2g/L CI-,CAT activities were the lowest treating with 3g/L CI-.Comprehensive analysis of experiment,2g/L Cl-was threshold for foxtail algal tolerance.2.Effect of Cl-and heavy metal complex stress on stress-resistance physiological index of Foxtail algal.Effects of Cl-,Pb2+ and Cu2+ complex stress on physiological index of foxtail algae was studied in 28 d cultivation.Foxtail algal biomass was the lowest and SOD activities reached the maximum,for which were treated by Cl-2g/L+Cu2+25mg/L+Pb2+100mg/L,POD highest activity,under four composite stress,POD activity decreased;CAT activity is higher than the control,the Cl-2g/L+Cu2+25mg/L+Pb2+50mg/L treatment under reached the highest activity.In the Cl-2g/L+ Cu2+ 50mg/L+Pb2+50mg/L treatment,MDA content reached the highest Foxtail algal by the maximum injury degree.In the Cl-2g/L +Cu2+50mg/L+Pb2+50 mg/L treatment,Foxtail algal had highest glutathione content.The results showed that high concentrations of Cu2+ has greater damage on Foxtail algal,adding more Cl' ion increased Cu damage to,but there is no obvious effect on the Pb.3.Complex stress of Cl-and heavy metals on Foxtail algal and its effects of heavy metal accumulation.Through the cultivation of 28d,the effect of Cl-?Pb2+?Cu2+ and their complex stress on the heavy metal enrichment of Foxtail algal were investigated.Single treatment with the same concentration of Pb and Cu,after added the Cl-,the Cu content of Foxtail algal was drop,the maximum enrichment is 6.53mg/g,the Pb content of Foxtail algal was rise,the maximum enrichment was 56.37 mg/g.Experimental results shown that Cl-may inhibit the absorption of Cu,but stimulates the Foxtail algal to absorb Pb.Compound treatment with the same concentration of Pb and Cu,after added the Cl-,the Cu content of Foxtail algal was not too different,the Pb content of Foxtail algal was rise,experimental result show that Pb may stimulates the absorption of Cu.Single treatment with the same concentration of Pb and Cu,after added the Cl-,migration rate of Foxtail algal was increased,the highest reached 75.2%,81.45%.Compound treatment with the same concentration of Pb and Cu,after added the Cl-,migration rate of Foxtail algal was increased,the highest reached85.03%?84.93%.Experimental result show that Cl-may stimulates the Transport capacity of heavy metals of Foxtail algal.4.Effects of Pb and Cu tailing leaching liquid on Phytoremediation efficiency by Foxtail algalThe seedlings were cultivated in 28d,study on the tailing leaching liquid accumulation of heavy metals and Phytoremediation Efficiency by Foxtail algal.Under the different concentration of Pb and Cu tailing leaching liquid,Foxtail algal absorption of Pb and Cu can reach 0.56 mg/g?1.54 mg/g;Migration rate of Foxtail algal was increased,the highest reached 97.53%?86.1%.Removal rate of heavy metal in tailing leaching liquid of Pb and Cu can reach 91.86%and 95.36%.The results of the experiment show that Foxtail algal had high enrichment factor to the heavy metal like Pb and Cu,Foxtail algal also had good repair capacity to the contaminated water by the heavy metal like Pb?Cu,and have a better application prospect.