Synthesis And Properties Of Fluorescent Sensors For Hg²⁺ And Cu²⁺

With the development of technology and the improvement of living standards, human beings pay more attention on the environment and their own health. Nowadays, the metal ions exist in every corner of our lives. Heavy metal ions that can be a threat to human's survival and natural ecological system, while some trace metal elements play a key role on human physiology.This paper selects fluorescence imaging technology, which has many advantages, such as simple and real-time monitoring, to examine metal ions. Then synthesis routes were proposed through conducting relative literature research. Finally, two excellent probes were obtained and a series of research were going on.The target probe CR-L was obtained through a reation with the coumarin derivatives and 5-amino dimethyl phthalate. It has the specificity for Hg+. After added with Hg2+, its fluorescence intensity could go up to almost 23 times than before. The probe can be applied in normal cellular physiological environment because of its stability in a wide pH range. The minimum level of its detection can be achieved at ppb level. And the probe CR-L was confirmed by the single crystal training test, UV-vis spectrum and fluorescence spectrum. After added with Hg2+, C=N bond of the molecular CR-L broke down, so that its coumarin fluorophore fluorescence can be released out. Then its fluorescence intensity was significantly enhanced. Hela cells were selected for cell imaging experiments. Results show that the probe can be successfully applied in living cells.The target probe FR-L was obtained through a reation with the fluorescence derivatives and 5-amino dimethyl phthalate. It has the specificity for Cu2+. After added with Cu2+, its fluorescence intensity can be significantly quenched. And the probe FR-L was confirmed by the single crystal training test, UV-vis spectrum and fluorescence spectrum. Results show that the probe FR-L has a specific recognition to Cu2+ and its fluorescence intensity can be significantly quenched at 510 nm after added with Cu2+. While in the presence of other metal ions, its fluorescent signal will not be interfered.