The Preparation And Sensing Property Studies Of Organic Small-Molecule Fluorescent Probes

Recently, the fluorescence molecular probe because of its high sensitivity, good selectivity, low cost, and convenient use, have received extensive attention of the scientific researchers. Through the design, synthesis of ideal fluorescent molecular probes, thus can reach to the anions, metal cations and neutral molecules of analysis and detection. Among various kinds of luminous materials, fluorene and its derivatives are of high thermal and light stability, which are simple and easy to synthesize and modify. And it has a wide energy gap. So it is a common hair blue material. This thesis devotes to fluorene class group as the parent and pyridine derivatives as identification reactive. The two kinds of new type of metal cation fluorescent molecular probes were designed and synthesized, which achieved practical application value of the results of the study. Main content summary is as follows.A novel diphenyl fluorene fluorescent molecular compound modified by N-(pyridine-2-ylmethyl)amine was designed and synthesized. The structure of the molecule was confirmed by infrared spectrum, mass spectrometry, nuclear magnetic resonance hydrogen spectrum. The recognition of the probe compound towards 15 different metal cation was studied through the ultraviolet absorption spectrum and fluorescence spectrum. The research shows that the probe compound has good recognition ability about Hg2+and Cu2+in DMF-water (1:1, v/v, pH=7.0) of the solvent system. And the binding of stoichiometric ratio are both 1:1. When the ligand is used to identify Cu2+, the binding constant is high and the anti-jamming is strong. And in a certain range, with the change of the concentration of Cu2+ it has good linear relationship, which can be used for qualitative and quantitative detection in the environment.A new type of diphenyl fluorene fluorescent molecular compound modified by N,N-bis(pyridine-2-ylmethyl)amine was designed and synthesized. The structure of the molecular was confirmed by infrared spectrum, mass spectrometry, nuclear magnetic resonance hydrogen spectrum. The recognition of the probe compound towards 15 different metal cation was studied through the ultraviolet absorption spectrum and fluorescence spectrum. In DMF-water (1:1, v/v, pH=7.0) of the solvent system, the probe compound has good recognition ability about Ni2+ and Cu2+. The binding ratio are 1:1 and 1:2 respectively. When the ligand is used to identify Ni2+, the binding constant is higher and the anti-jamming is stronger than Cu2+. The fluorescence spectrum shows that the relative fluorescence intensity of probe compound with the change of the concentration of Ni2+ and Cu2+ has a linear relationship within a certain range, which accords with Stern-Volmer quenching equation.