| Zein as a kind of natural and amphiphilic polymer materials, has extensive research and application. Zein is a kind of mixed protein and contains most ?-zein. In this study, the self-assemble of hydrophobic proteins into amyloid nanofibrils was studied, using ?-zein as a model protein. The self-assembled morphologies of ?-zein were determined by hydrophobic-hydrophilic characters of both the protein and the solvent, and the inhibitory effect of hydrophobic interactions and ethanol in fibrillation of hydrophobic proteins was proved. The fibrillation of ?-zein shows great potential in serving as bio-based gels or reinforced fillers in food, cosmetic and pharmaceutical applications.By adding nanocrystalline cellulose crystal into zein ethanol solution observed diluting ethanol concentration of the ethanol solution can inhibit self-assembly process into a spheres, prevented ?-zein generating spheres. On hot hatch self-assembled nano crystalline cellulose fiber had a promoting effect, promoted generating branched chain nanofibers. At the same time, crystal fiber could improve the modulus of ?-zein film increase membrane brittleness and fracture tensile, reduce elongation at break.We prepared curc?min-loaded ?-zein spheres by dilution method and hot hatch method, the experiments verified ?-zein and curc?min combined by hydrogen bond force. We found that hot hatch ?-zein spheres drugs slow-release effect is the best, release quantity is good by comparing the preparation of curc?min-loaded ?-zeins. |
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