Effects Of Turbulent Dissipation On The Monosaccharide Composition Of Exocellular Polysaccharide Of Typical Alage And It's Biomechanical Characters

The study around the content and the monosaccharide composition of exocellular polysaccharide of typical alage and it's biomechanical characters to carry out the research. And focused on Aphanizomenon flos-aquae culturing individually and conducted six experiments to research the effects of turbulent dissipation on the growth of Aphanizomenon flos-aquae.And conducted six experiments on the basis of extraction,isolation, purification Exocellular Polysaccharide of Aphanizomenon flos-aquae in order to quantitative research the effects of turbulent dissipation on the the Exocellular Polysaccharide of Aphanizomenon flos-aquae, and research the effects of turbulent dissipation on micro-mechanics behavior of Aphanizomenon flos-aquae in the single cell level by AFM Force spectrum technology.Six experiments were performed in culture condition by changing the rotational speed under a certain light(50?mol photos m-2 s-1, 12h:12h)and temperature conditions(25?).The results showed that:?The turbulent dissipation has significant effects on Aphanizomenon flos-aquae growing when the turbulent dissipation rates was 0.00151 ? 0.00663 ? 0.02259 ?0.05058m2/s3 can promote the growth of Aphanizomenon flos-aquae,the absorption of Aphanizomenon flos-aquae on carbon and nitrogen and phosphorus,the viable cell of Aphanizomenon flos-aquae and the diameter of single Aphanizomenon flos-aquae cell, and all of these positively related to turbulent dissipation intensity and the turbulent dissipation rates was 0.08010m2/s3 will inhabit the physiological activity of Aphanizomenon flos-aquae.?when the turbulent dissipation rates was lower than 0.05058m2/s3 can promote the secretion of EPS of Aphanizomenon flos-aquae,this is mainly because the total content of EPS of Aphanizomenon flos-aquae positively related to the cell number of Aphanizomenon flos-aquae.The content of bEPS of single Aphanizomenon flos-aquae cell was decreased when the turbulent dissipation rates was 0.05058m2/s3,which is not fully verified that the higher intensity of turbulent dissipation,the thinner sublayer it was.The monosaccharide composition was not been detected when the turbulent dissipation rates was 0m2/s3?0.00151m2/s3?0.00663m2/s3, however the monosaccharide composition respectively were fructose, mannose and fructose, mannose when the turbulent dissipation rates was 0.02259m2/s3?0.05058m2/s3?0.08010m2/s3.?We found out that the change of cell mechanics properties is more sensitive than the change of cell morphology by using AFM to scan the morphology of Aphanizomenon flos-aquae cell and determine the cell mechanics properties of Aphanizomenon flos-aquae.With the increase of turbulence intensity of disturbance, cell size of Aphanizomenon flos-aquae is bigger and it's cell morphology is largely unchanged.The alage cell is cylindrical,solid and has clear outline and smooth surface.But when the turbulent dissipation above a certain threshold, the morphology of Aphanizomenon flos-aquae cell has a obvious change, some of them is not cylindrical,solid and has not clear outline and smooth surface.With the increase of turbulence intensity of disturbance, the Yang's module is gradually increasing, but when the turbulent dissipation effect is bigger,the Yang's module of Aphanizomenon flos-aquae cell is decreasing.The research lay the experimental basis for further understanding the mechanism of resistance to deformation and keep stable structure of alage cell in the constitution close to nature, which play an important role on resisting and controlling eutrophication,have the important academic value.