Kinetics Studies On Oxidative Synthesis Of Disulfides By Pt(?) Complex And Synthesis Of Disulfides In Peptides By Solid Supported Pt(?) Complex

Intramolecular disulfide bonds exist in a variety of peptide-based drugs and play very important functions in biological processes. Oxidation of thiol containing compound for synthesis of intramolecular disulfide bonds is the most common method. However, there are some disadvantages such as low yield, long reaction time. Therefore, efficiency synthesis of intramolecular disulfide bonds is a research hotspot in recent years. Pt??? complexes can efficiently and quantificationally oxidize thiol-containing compound to intramolecular disulfide bonds. But it is very expensive, which stunted its application in disulfide bond formation.In our work, a Pt??? complexes trans-[PtCl₂?en??heda?]Cl₂ was synthesized and characterized by elemental analysis, 1H NMR, high resolution mass spectrometry, X-ray crystallography diffraction. The reaction mechanisms of oxidation of3,6-dioxa-1,8-octanedithiol and dithiothreitol by the Pt?IV? complex were investigated by a rapid scan spectra, product analysis, stoichiometric ratio, and kinetics studies.3,6-dioxa-1,8-octanedithiol and dithiothreitol were oxidized to disulfides. They are all second-order reactions.Magnetic nanospheres supported Pt??? complexs were synthesized in this work and were characterized by fourier transform infrared spectroscopy?FTIR?, elemental analysis?EA?,inductively coupled plasma mass spectrometry?ICP-MS?, transmission electron microscopy?TEM?, vibrating sample magneteometry?VSM?, X-ray photoelectron spectroscopy?XPS?. The obtained magnetic nanospheres oxidize peptide?Ac-CPFC-NH₂? and reduced vasopressin to intramolecular disulfide bonds. The reactions are investigated by HPLC. The magnetic nanospheres supported Pt??? complexs react with polypeptides well and can oxidize peptides to intramolecular disulfide bonds effectively.