Preparation Of Semiconducting Polymer Dots And The Application In Cell Labling

As a new type of labeling materials, the ultra-bright fluorescence semiconductor polymer dots(Pdots) are very inspiring due to their excellent optical performance,biocompatibility, photostability etc. Therefore, they are widely studied by researchers from various fields such as chemistry, biology, materials and medical science etc. In this research,we used the poly[{2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylphenylene)}-alt-co-{2,5-bis(N,N'-diphenylamino)-1,4-phenylene}](DB-CN-PPV) and poly [(9,9-dioctylfluorenyl-2,7- diyl)-co-(1,4-benzo-{2,1,3}-thiadiaole)](PFBT) as initial materials, to fabricate low-toxic semiconductor polymer dots by reprecipitation method. Besides, this work also explored the relationship of solvent purity and particle size of PFBT Pdots, and the optical physics of Pdots. The absorption spectra shows red shift, while the emission spectra presents blue shift with the particles size increasing.In addition, this work also presented the size and morphology character of DB-CN-PPV Pdots using Scanning Electron Microscope, the result of nanosphere DB-CN-PPV Pdots size is 20~30 nm. Furthermore,we compared the fluorescence brightness of DB-CN-PPV Pdots and conventional Qdots655 by Single-particle imaging and analyses indicate the brightness of DB-CN-PPV Pdots is four times brighter than that of the Qdots 655. These DB-CN-PPV Pdots have been used to label breast cancer cells MCF-7. The experimental results show that the DB- CN- PPV is a kind of excellent fluorescence labeling materials. It has shown great potential for practical application in the fields of biology.