Research On Detection Technology And Application In Technique Of Spectroscopy And Mass Spectrometry Of Typical Edible Oil

It is well known that governments and organizations around the world have attached great importance to the food safety issues, and many policies and regulations about the using of edible oil were enacted. Meantime, environmental and food safety issues caused by edible oil become increasingly serious, which will affect the safety of human life. Therefore, it is inevitable trend to develop new edible oil detection method combining with modern science and technology. This work has been carried out under the above-mentioned background and carries out to a series studies on the basis of laboratory. This paper mainly studies the edible oil detection method of color additives, pesticides, plasticizers pollution problems by spectroscopic and mass spectrometry technology, and the quantitative analysis mathematical models are established about edible oil based on spectroscopic and mass spectrometry technology, exploring a practical, fast, accurate and efficient detection methods. In this paper, the research results have important implications for in-depth study of edible oil detection technology.The main innovations and research contents of this thesis are as follows:(1) Ii is put forward to detect Rhodamine B in capsicol based on the UV-visible absorption, The second-derivative UV spectrophotometry is applied to the determination of Rhodamine B(RB) in capsicol. Based on the spectrophotometry of the capsicol-RB mixture, and by calculating the evaluation parameter value of recovery, detection limit and limit of quantification and original absorption spectrometry were compared. The prediction models of Rhodamine B in capsicol were obtained according to the experimental results through the regression analysis, and the correlation coefficient of the model is 0.997,verifying that the second-derivative UV spectrophotometry method in this paper is feasible to detect the RB in capsicol, it has higher accuracy and sensitivity With comparing the original absorption spectrometry method.(2) Fluorescence spectrophotometry was applied to the determination of pesticide residue(carbendazim, metiram, chlorothalonil) in edible oil(rapeseed oil,Olive oil, peanut oil). Based on the fluorescence spectra of the oil-pesticide mixture, the prediction models between fluorescence intensity and pesticide content can be obtained. Meantime the model function was evaluated and analyzed including these aspects of recovery rate, detection limit and the limit of quantification, etc. The analysis results show that the method in the linear range had a good function relationship.In the model function of carbendazim: the best average recoveries was 99.26%, The minimum detection limit of the method was 7.07×10-4mg/ml, the limit of quantitation was 21.42×10-4mg/ml, the relative standard deviations was 3.28%.In the model function of metiram: the best average recoveries was 100.90%, The minimum detection limit of the method 79.28×10-4mg/ml, the limit of quantitation was 28.12×10-4mg/ml, the relative standard deviations was 1.52%. In the model function of chlorothalonil: the best average recoveries was 100%, The minimum detection limit of the method was 4.87×10-4mg/ml, the limit of quantitation was 14.76×10-4mg/ml, the relative standard deviations was 2.27%. It was verified that the fluorescence spectrophotometry method in this paper was feasible to detect the pesticide residues in edible oil. This study provides a new way for the detection of pesticide residues.(3) Determination of PAEs in Edible Plant Oils, Sample preparation involves a solid phase extraction(SPE), and a gas chromatographic-mass spectrometric method for determining PAEs(DBP?DEHP?DINP)in edible oil have been developed.The experiments showed that the method in the linear range had a good linear relationship,and the correlation coefficients are basically over 0.99. The detection limits of DBP?DEHP and DINP was 0.01?0.02 and 0.02mg/L, respectively. The recovery rate of the method was above 90%, the relative standard deviation of the method was less than 10%.To establish a method by studying PAEs in edible oils, it will further provide the basis for the PAEs safety evaluation.This study provides a new way for the detection of edible oil.