The Spectroscopic Properties Study Of Novel Rhodamine B-based Fe3+ Probes And Their Cell Imaging Applications

The content of Iron is in the second place of all the metal elements on Earth, which plays a very important role in the organic life processes. The Iron involves in the blood oxygen transport, proton transfer, the synthesis of DNA and RNA, the regulation of intracellular osmotic pressure and maintenance of acid-base balance, etc., at the same time constitutes the cell metabolism enzymes essential elements. Both the macroscopic iron and some traces even the metallic iron ions play a key role in the process. Therefore, the detection of iron ions is widespread concerned by medical and biochemical industry. In this paper, the chromophore of rhodamine, using its lactam ring having a spiro "OFF-ON" mechanism, designed and synthesized a series of novel molecular fluorescent probe ?L1-L6? and characterized using spectral properties of fluorescent probes selective identification Fe³⁺, examining the application of the probe in living cells. The main contents include:1.Design and Synthesis of Novel Molecular fluorescent probes L1-L6 and characterized. Based on the oxygen mixed anthracene ring of "OFF-ON" mechanism, use the rhodamine B as the starting material by condensation reaction thus obtain a new series of fluorescent probes L1-L6, with ¹H NMR,¹³C NMR, IR, HRMS-ESI and X-rays to probe the structure of the compounds were confirmed.2.Study the fluorescent probe recognition performance of L1-L6. The spectral properties of the probe ?including selectivity, competition, the concentration gradient, Job curve and PH effects? and UV spectrum ?including selectivity, the concentration gradient?, which indicates that there is good relation between the probe and Fe³⁺. Job confirmed the probe and the Fe³⁺ are combined by 1:1. pH stability tests show that in the pH range of 4-7, the pH of the solution virtually has no interference to the emission intensity of fluorescent probes. The detection of Fe³⁺can be realized under physiological conditions.3.Study the probe complexation Fe³⁺application in living cells. Research the probe L1-L6 cytotoxicity to the cells by MTT method, the results showed the lighter probe cytotoxicity. Probe L1-L6 recognizes mouse nerve cells ?PC-12? in the Fe³⁺imaging experiments showed that probe biological systems detected Fe³⁺ has the ability to identify efficient selection, it is closely combined with the target molecule, can be in a biological environment Fe³⁺ on the qualitative and quantitative detection, low toxicity, penetration ability, does not affect the guest molecule structure and biological activity, with good anti-interference ability and other characteristics, has important significance for live cell detection of Fe³⁺.