Black Tea Increases Hypertonic Stress In Caenorhabditis Elegans

As the world's most popular tea, black tea health functions attracted widespread attention. This study investigated the protective effect of tea under osmotic stress conditions of Caenorhabditis elegans. The results are as follows:After 500mmol/L Nacl osmotic stress 12hr, water, black tea extract can effectively improve the survival rate of Caenorhabditis elegans. Compared with the control group,50mg /L,1 OOmg/L and 200mg/L black water extract treated group survival rates were increased by 22.62%,34.70% and 26.97%, respectively, to extend the length of the 4.39%,17.94% and 11.87%. In addition, black tea can increase Caenorhabditis elegans in Na2SO4,Kcl,Licl 3 and the salts penetrate stress survival. Description, black tea can improve the penetration of Caenorhabditis elegans anti-stress ability and independent of the ionic salt species.Black anti-stress effect penetration and p38 MAPK signaling pathway is not relevant. After 48hr tea processing, as the wild-type N2, black tea aqueous extract can also extend unc-43, nsy-1, pmk-1 sek-1 gene and mutant lines AU3, KU4, MT205 and KU25 survival. In addition, black tea processing 48hr vivo Caenorhabditis elegans unc-43, nsy-1 and pmk-1 gene showed no significant change.Black anti-stress effect and penetration WNK/GCK VI signaling pathway. Osmotic stress, water extract of black tea can significantly increase wnk-1 and gck-3 gene mutation strain RB879 and EV232 survival. Similarly, after tea processing 48hr, Caenorhabditis elegans wnk-1 and gck-3 expression levels and significantly increased.When the tea water extract can significantly reduce the penetration of stress proteins in the C. elegans mutants IG274 nlp-29::GFP expression, but does not reduce AM 140 nematode PolyQ35::YFP abnormal protein aggregation.These results indicate that black tea can increase WNK/GCK VI signaling pathway in Caenorhabditis elegans to improve resistance to penetration stress ability. Since Caenorhabditis elegans highly conserved signaling pathways in vivo, this study can improve the higher animals black osmotic balance the ability to provide important reference, which is of great biological significance for further reveal black tea health effects.