Enzyme-Immobilized Nanotube-Chitosan Membranes With Sustainable Biocatalytic Activity

The key of bio-catalytic technologyis that under the conditions of green and sustainable chemical manufacturing can be discussed,and the immobilization of enzymes is as a means of economic efficiency.Immobilization is the key technology of enzyme used in industrial process,especially for green sustainable energy and chemicals from biomass by catalytic conversion.The influence of different immobilization methods for its function also cannot be ignored.As a result,the enzyme fixed on a carrier can strengthen the storage performance and operational stability,and the immobilized enzyme consistent with the free biological enzyme,its stability and recycling use should be strengthened.The more profound advantage of immobilized enzymes is that theenzyme isless likely to be contaminated with the product.In recent years clay nanotubes is a special chemical structure with natural silicate minerals,widely distributed in china.Due to its low price,the structure is widely used in biological and chemical industry.Used as a nano-scale reactor as well as the carrier and so on.Chitosan is a polysaccharide extracted from shrimp shells,and is a natural world existence alkaline polysaccharide.Chitosan also has the characteristics of abundant resources,and have abundant hydroxyl and carboxyl,chitosan is so easy to make film or gel widely used in food,medical and other fields.Baced on the understanding of natural substance,this paper developed a simple and effective method,by mixing chitosan solution with nanotube / lipase,preparation for bio-film structure with enzyme activity.The lipase was loaded into the lumen of clay nanotubes by electrostatic interaction.Clay nanotubes containing lipase are dispersed in the chitosan solution and the mixture is used for coating the film.The enzyme activity of chitosan clay nanotube film showed pH stability,temperature stability and long-term storage stability in the catalytic degradation of oil and grease.A membrane structure with biological enzyme catalytic activity was prepared by using a simple and sustainable material clay nanotube,chitosan,and a biological enzyme molecule with antibacterial properties.The obtained clay nanotube / enzyme hybrid chitosan membrane showed good ability to catalyze lipid degradation,and also showed good stability and reuse performance.Secondly,the cationic lysozyme adsorbed on the outer surface of the nanotube,and can play the role of antibacterial and anti fouling.