Construction And Application Of Biosensor Based On Electrospun Nanofibers

Recently, biological sensing technology, as a frontier technology, has attracted considerable attention. They have been applied in various kinds of fields, including environmental detection, clinic diagnostics, life sciences, immunoassays and so on, due to their high sensitivity, simplicity, versatility, stability, rapidity, easy controllability. Nowadays, with the rapid development of technology, the hot research and trend is to establish the bisensors with practicality, availability, simplicity, rapidity, environment friendly, high selectivity and sensitivity. Therefore, in order to develop the sensor with good properties, electrospun nanofibers were used to establish the electrochemical and electrochemiluminescent sensors in this research. The electrospinning (ES) is a rapidly developing and low cost technique for the mass production of nanofibers with controllable diameter in nanometer scale. Moreover, various nanometer material functional nanofibers have attracted considerable attention and made them highly attractive to different applications. Therefore, promoted by the development of material science and nanotechnology, we not only used electrospining technique to synthesize various functional nanofibers, but also employed these nanofibers to establish the electrochemical and electrochemiluminescent sensors.Therefore, this research has three parts:1. In this part, simple molecular recognition sites for formaldehyde were designed on electrospun polymer nanofibers. In order to improve the conductivity of the electrospun polymer nanofibers, carbon nanotubes were introduced into the resulting nano fibers. By employing these functionalized nanocomposite fibers to fabricate a biomimetic sensor platform, an obvious change caused by recognition between recognition sites and formaldehyde molecules was monitored through electrochemical impedance spectroscopy2. A new biosensing platform based on electrospun carbon nanotubes nanofibers composite, which enabled strong electrochemiluminescent emission of peroxydisulfate, was firstly developed for immunoassay with favorable analytical performances, and then was utilized to evaluate the interaction between antibody and antigen in vitro.3. A label-free electrochemiluminescent immunoassay protocol was fabricated based on magnetic nanoarchitecture for sensitive detection of aflatoxin B1. It was constructed by using magnetic control technology to immobilize the magnetic nanofibers on carbon nanohorns matrix. Therefore, the proposed strategy with strong specificity, high sensitivity, low cost and fast detection provided a new way for bioanalysis and effectively improved the efficiency of analysis.