| Chaenomeles speciosa is declared special products for Xuancheng,with medicine and food homologous,contains a variety of useful ingredients for the human body,such as flavonoids,protease,terpenoids and so on.The conten t of flavonoids in the Chaenomeles speciosa is rich,and it has good antioxidant activity,which is free radical scavenging and interrupt chain reaction.Therefore,the development of natural antioxidants with Chaenomeles speciosa as raw materials is a new way to tap its deeper value.Purpose: Extraction of Chaenomeles speciosa flavonoids with organic solvent heat reflux method.The extraction process was optimized to prepare the crude extract of flavonoids;the crude extract was purified by macroporous resin column separation.The purification process was optimized to prepare the purified product of flavonoids.Comparison of antioxidant activity of crude extract and purification of flavonoids through in vitro antioxidant experiments.The effect of crude extract and purified product on fat oxidation of meat samples was studied using TBA as an index.Methods: Design extraction times,ethanol volume fraction,liquid to material ratio,reflux temperature,time of the five single-factor experiments,using ethanol as extractant.Do orthogonal designs based on single factor experiment results.The optimum extraction process of flavonoids was optimized to prepare crude extract of Chaenomeles speciosa flavonoids.Select D101,AB-8,D1300,HP-20 macroporous resin,select the appropriate resin by static adsorption and desorption experiments.Optimization of flavonoid purification process of Chaenomeles speciosa by dynamic adsorption and desorption experiment.The antioxidant activities of the crude extract and the purified product of the flavonoids of Chaenomeles speciosa were compared with the total reducing power,· OH removal force and O2?· scavenging activity;Adding flavonoids to fleshy samples and taking TBA(Thiobarbituric acid)as an indicator,analyzing of the effe ct of fat oxidation on meat samples in 14 days.Results:(1)The optimum extraction conditions were as follows: ethanol volume fraction is 80%,liquid ratio is 15:1,temperature is 80?,time is 4.5h,extraction times 2 times.Primary and secondary factors: ethanol volume fraction> temperature> time> liquid to mate rial ratio,the extraction rate of this process is 0.639%,RSD = 0.017.(2)Through the static adsorption and desorption experiments,HP-20 macroporous resin was used to purify the crude extract of Chaenomeles speciosa,the optimal conditions for dynamic adsorption and desorption experiments were as follows: The injection volume is 1mg/m L,the injection flow rate is 2m L/min,the maximum injection volume is 300 m L,the ethanol volume fraction is 80%,the elution flow rate is 1m L/min,the eluent volume is 90 m L and the elution rate is 83.7%.The purity of flavonoids was increased from(5.13±0.27)% to(29.20±0.74)% and the purification rate is(569.57±21.53)%.(3)The total reducing power,·OH scavenging ability and O2?· scavenging activity of the purified flavonoids were higher than that of the crude extract.Crude extract and purified fatty oxidation have a certain degree of inhibition in 14 days,the inhibitory effect of the purified substance is significant(P <0.05).Nine groups of purified and VC compound formulas have an inhibitory effect on fat oxidation.Formula six has the best effect,and the effect is better than single addition.Afte r 14 days,the components L*,a* and b* of flavonoids were significantly changed(P <0.05),and the values of L* value was decrease,a*and b * value were increase.Conclusions:The extraction process of antioxidant activity was optimized by ethanol refluxing;Selected HP-20 macroporous resin to purify,the purity of flavonoids from Chaenomeles speciosa was improved from(5.13±0.27)% to(29.20 ± 0.74)%.The antioxidant activity of the purified product of Chaenomeles speciosa flavonoids is significant.The inhibitor y effect of fat oxidation is effective.The mechanism of its impact on the meat color of is not clear,need further exploration. |
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