Quartz Crystal Microbalance Sensor Based On Molecularly Imprinted Polymer For Patulin Determination

Patulin?4-hydroxy-4H-furo[3,2-c]pyran-2?6H?-one?is a fungal secondary metabolites.Some of these species are responsible for therotting of fruits,and it will still exist after manufacturing process.There is a great potential danger to human body because patulin has teratogenic,carcinogenic,mutagenic and immune toxicity and genetic toxicity,Therefore the development of a rapid and sensitive method for the determination of patulinis is highly desired.In this study,a novel imprinted material using oxin as template molecule,3-aminopropyltriethoxysilane?APTES?as functional polymer,tetraethoxysilane?TEOS?as cross-linker according to optimization for patulin recognition was synthesized in sol-gel process.The adsorption performance of the prepared material was evaluated by adsorption equilibrium binding experiment,adsorption kinetics experiment and Scatchard equation analysis.And then,the developed material was used on the surface of quartz crystal platinum electrode as sensitive membrane and immobilized to fabricate a molecular imprinted quartz crystal?MIP-QCM?sensor for patulin detection.The result demonstrating a high mass transfer velocity and saturated adsorption capacity of the developed material.Through the evaluation based on frequency change,the developed sensor exhibited a detection limit of 3.1×10-3 ?g mL^-1 and concentration linear range from 7.5×10-3 ?g mL^-1 to 6×10-2?g mL^-1 with good selectivity and the selectivity coefficient was 3.82.It also showed good reproducibility and long-term stability.The developed sensor was applied for the detection of patulin in apple juice,pear juice and haw flakes.The traditional LC-MS method was used for validation.The recoveries of PAT in food were in the range of 76.9%-91.3%with the RSDs rangingfrom 1.2%to 8.4%.In addition,the result obtained using the established MIP-QCM sensor showed great relative?correlation coefficient,0.9899?to the result of LC-MS method,indicating the accuracy of the developed method in practical application of patulin determination.