| Recently,with the upsurge of golbal anti-smoking campaigns,development of less harmful cigarette has become a common perspective of tobacco industry and the safety of the additives has attracted increasingly great attention from all walks of life.The natural plants’extract,which are rich of the active ingredients and aromatic components,can be used as the additives of cigarettes with using traditional Chinese medicine theory.In this study,natural plants of zhejiang province,which have been used as a humectant in cigarette,have been chosen by assessment of smoking sensory analysis.one of plants will be chosen for further research concerning the technology of extraction for carbohydrate,characteristic comparison of humectants and active ingredients’ research.The main work and results were as follows:Thirty-seven natural plants of zhejiang province,which have the antitussive and expectorant effect,have been chosen with using traditional Chinese medicine theory.The measurement of carbohydrate content of these plants shows huge difference between 1%to 50%.Five natural plants’ extract shows good smoking sensory analysis in cigarette.One of them was Chuanminshen violaceum.To optimum extraction conditions of ultrasound-assisted technology of carbohydrate from Chuanminshen violaceum by response surface methodology.The optimum ultrasound-assisted extraction conditions were as follows:the ratio of water to sample 21:1,ultrasonic power 530W,extraction time 30min.Under this condition,the yield of Chuanminshen violaceum of carbohydrate was 55.37%.Campared with direct-heating extraction rate was improved to about 48%.With distilled water and propylene glycol as contral,The different components of Chuanminshen violaceum plays a good mositurizing effect.The polysaccharide of Chuanminshen violaceum,which can reduce the loss of tobacco moisture in the early stage,shows better mositurizing effect than other components of Chuanminshen violaceum.Compared with the traditional humectant propylene glycol,The polysaccharide of Chuanminshen violaceum still have a certain gap.The polysaccharide fractions from water extract of Chuanminshen violaceum were isolated and puried using DEAE Sepharose F.F.and Sephacryl S-300.Two water-soluble polysaccharide were obtained.UV scanning indicated that they contained no protein and nucleic acid.HPLC producing indicated their molecular weight were 4.02×104Da、1.97×103Da、2.05×106Da、1.22×104Da、1.87×103Da,respectively.In addition to a large molecular weight polysaccharide,the other four polysaccharides were small molecular weight,which can play better mositurizing effect than large molecular weight polysaccharide. |