Study On Extraction Of Ovum Oil Of Rana Chensinensis And Its Hypolipidemic Activity

Ovum oil of Rana chensinensis was extracted by Soxhlet extractor method and supercritical carbon dioxide method in this paper, and the extraction effect of ovum oil of Rana chensinensis was compared and analyzed. After methyl ester of the ovum oil, the types of fatty acids of the ovum oil was determined by GC-MS technique with the optimal methyl esterification method. The high fat model rats were drenched with the ovum oil of Rana chensinensis. Content of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglyceride of the hyperlipidemia rats model were menstruated in rats’ blood serum. The activity of reducing blood lipid of the ovum oil was determined. Finally, the method of microcapsule embedding was used to embed the ovum oil of Rana chensinensis, achieved good results and calculated the embedding rate. Its water solubility was determined that the ovum oil of Rana chensinensis was embedded. The main results are as follows:Ovum oil of Rana chensinensis was extracted by Soxhlet extractor method to obtain the results through statistical analysis. The statistical analysis showed that the optimum extraction conditions(extraction temperature 60.62℃, extraction time 3.16 h, the ratio of ovum oil of Rana chensinensis and extraction solvent 1:8.91) led to an amount of ovum oil of Rana chensinensis(19.3%).Ovum oil of Rana chensinensis was extracted by supercritical carbon dioxide extraction method to obtain the results through statistical analysis. The statistical analysis showed that the optimum extraction conditions(extraction temperature 36℃, extraction time 2h, extraction pressure 20 MPa, ovum oil of Rana chensinensis size 40) led to an amount of ovum oil of Rana chensinensis(19.3%).Then the fatty acids of ovum oil were analyzed. The methyl ester of ovum oil was compared through different methods(H2SO4 methanol method, KOH methanol method and BF3 methanol method), and the optimal reaction conditions were selected. Quality of 3 kinds of esterification reaction was selected by orthogonal experiment. 14% BF3-methanol method was for the final choice of methyl esterification. 19 kinds of fatty acids of ovum oil were detected by GC-MS Methods.Finally, the high fat model of rats was established. The hyperlipidemia rats model was randomly divided into four groups, including simvastatin group, group of ovum oil of Rana chensinensis, honey group and self-healing group. Different drugs were drenched consecutive 14 days to verify the effect of ovum oil on triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol in serum of rats. The results proved that the ovum oil of Rana chensinensis has the effect of lower blood lipid and its effect is similar to that of Simvastatin. It can be used as healthcare product to provide application for masses of hyperlipidemia.Ovum oil of Rana chensinensis obtained by supercritical carbon dioxide extraction method was embedded into gelatin. The optimal embedding conditions(concentration of gelatin 4.5%, The ratio core and wall 1:5, concentration of emulsifier monoglyceride 0.3, temperature 50℃) led to embedding ratio 75%.