Study On The Cooperative Desulfurization With SRB And PSB In Light Anaerobic Microecological System

Sulfate reduction treatment technique is known as one of the most development desulfurization in twenty-first century, as we all known sulfate wastewater give harm to humans life more and more serious, for this case, we made four UASB anaerobic reactor, two sets of increased light, the other two groups do not add light, high concentrations of experiment simulated sulfate wastewater is pumped into the groups everyday, the four rreactors is cultured in the incubator of 35?, by measuring the effluent COD, SO₄^2- and p H.Analyzing running comparison of light and non-light system.By constantly increasing the wastewater load, set influent COD value is 1000mg/L 5000mg/L, when the load is setup to 5000mg/L, measured the values of the four reactor effluent COD, p H, and sulfate removal, light reactor higher than the non-illuminated COD removal of 20% to 25%, four reactor effluent SO₄^2- removal are at a high level at around 85%-88%, light reactor 's p H is higher than the non-lighting,the valueis about 2.0 2.5.The COD concentration from 5000mg/L to 6000 mg/L, the 1 and 2 reactor COD removal efficiency is above 90%, 3 and 4 reactor COD removal efficiency is more than 60%; sulfate removal rate reached above 85%. When the Influent COD concentration was 7000mg/L, the light and non-illuminated reactors effluent SO₄^2-, COD and p H shows significant difference, the p H and the effluent COD removal of light reactor is significantly higher than the non-lighting reactor. When the influent COD was 10000 mg/L and SO₄^2- was 4000 mg/L, the light reactor sulfate, COD and p H decreased slightly but remained stable, rather than the non-lighting reactor shows no metabolism for COD and SO₄^2-. Description that the light on the reactor has significantly promoted the treatment of high concentration SO₄^2-, COD wastewater and p H stability.Screened out the efficient Strain from the light reactor and the non-illuminated anaerobic reactor,the efficient Strain can remove sulfate efficiently. Domesticated strains can tolerate higher concentrations of sulfate,named SRB1?SRB 2?SRB3 and SRB4. After several solid-liquid and solid-solid alternate screening, and ultimately selected 12 plants. Each system corresponding 3 plants, That is SRB1-2?3?4,SRB 2-1?2?5,SRB 3-4?5?6,SRB 4-2?3?4. For removal of sulfate of the 12 strains is higher than 80%.The tolerance to the high concentrations of sulfate is about 7500mg/L.Screened out the efficient Strain from the light reactor and the non-illuminated anaerobic reactor,the efficient Photosynthetic sulfur bacteria can remove Sulfide efficiently. Only in the light system can filter out this Photosynthetic bacteria. After three domesticated,we got domesticated strains that they can tolerate higher concentrations of sulfide. After several solid-liquid and solid-solid alternate screening,we finally got pure colonies that they can tolerate the concentration of S2- is 300mg/L.By Petri dish culture, Gram stain, scanning electron microscopy?culture studies were done aerobic and anaerobic?. Preliminary identification of the strain: by Gram staining experiments and found that they are gram-negative bacteria; by scanning electron microscope, the bacteria is the cell size is?0.2-0.4??m×?1.8-2.5??m, the rod shape. Under aerobic conditions wherein the light illumination anaerobic conditions and cultured bacteria cultures could grow but there are obvious differences.