Research On The Effect Of Two Organogels On In Vitro Simulating Digestion Of Edible Oil

Two different types of organogelation products had been observed in this issue.One was bonding mixture of ?-sitosterol and ?-oryzanol as the representatives of plant sterol;another was ethyl cellulose as a representative of complex carbohydrate.The purpose was to study the effect of plant sterols and ethyl cellulose on triglyceride enzymatic hydrolysis reaction in organogel-based oil systems in vitro model simulating gastrointestinal digestion.Variables included the content of organogels in organogelation oil products,the type of emulsifiers in organogelation oil products and the storage condition of organogelation oil products.The indicators included the release rate of free fatty acids(FFA),particle size,zeta potential etc.Results of effect of plant sterols on triglyceride enzymatic hydrolysis reaction in vitro model simulating gastrointestinal digestion showed that in vitro simulating digestion of edible oil was inhibited by plant sterols(2%-10%w/w).And the least influence on plant sterol margarine product was the sample with 6%(w/w)plant sterols.Moreover,it were the sample with 6%(w/w)plant sterols who had the least negative zeta potential(-56.7mV),which meant poor system stability.Besides,in different emulsifiers(including span 60,tripolyglycerol monostearates,mono-diglyceride and distilled monoglyceride),plant sterols had different influence on triglyceride enzymatic hydrolysis.The greatest impact on plant sterol margarine product was associated with the sample with distilled monoglyceride(release rate of FFA reduced from 55%to 50%).At 4? and 25?,as time goes on,the release rate of FFA of plant sterol margarine product showed a trend of first increase and then decrease.In the meantime,the plant sterols had greater impact on the margarine sample at 4? than on the sample at 25 ?.Results of effect of ethyl cellulose on triglyceride enzymatic hydrolysis reaction in vitro model simulating gastrointestinal digestion showed that in vitro simulating digestion of edible oil was inhibited by ethyl cellulose(2%-10%w/w).And the extent of inhibition was considered to be linear with the content of ethyl cellulose,expect the samples with 2%(w/w)and 6%(w/w)ethyl cellulose for less effect(release rate of FFA reduced from 49%to 45%and from 49%to 44%,respectively).Moreover,it were the sample with 6%(w/w)ethyl cellulose who had the least negative zeta potential(-52.1 mV),which meant poor system stability.Besides,in different emulsifiers(including span 60,tripolyglycerol monostearates,mono-diglyceride and distilled monoglyceride),ethyl cellulose had different influence on triglyceride enzymatic hydrolysis.The greatest impact on ethyl cellulose shortenings product was associated with the sample with tripolyglycerol monostearates(release rate of FFA reduced from 50%to 39%).At 4? and 25? as time goes on,the release rate of FFA of ethyl cellulose shortenings product showed a trend of first increase and then decrease.In the meantime,the sample at 4? had the wider range than the sample at 25 ?.With the increase of the extent of plant sterols and ethyl cellulose,hardness and gumminess increased,crystal behavior of samples became more fine and well-distributed,and water liquidity became lower.It made a contribution to inhibition on in vitro digestion.At the same time,it was also affected by different emulsifiers and storage condition.