Preparation Of Fluorescent Nanomaterials And Its Application In Fluorescence Analysis

Fluorescent nanomaterials are one of the popular materials of chemical and biosensing materials in recent years.This paper prepared several novel fluorescent nanomaterials including gold nanoparticles,carbon quantum dots and metal-organic framework materials?MOFs?and explored their fluorescence properties.The corresponding works were conducted as the follows:1.The preparation of nanoscale coordination polymers with core-shell nanostructures by using nanoparticles as template has attracted increasing interests owing to the convinently controlled size and morphology.In this study,Si O₂@Tb/GMP coordination polymers with core-shell nanostructures and nanoscale size was developed for superoxide?O₂·-?detection.It is of great significance for O₂·-detection for its important roles in regulation of biological function.The nanocomposite was produced by using SiO₂ as template to induce the growth of Tb/GMP on its surface.First,Tb3+was adsorbed on the surface of the SiO₂ nanoparticales by coordination.Then Tb/GMP shells could grow along the surface.The core-shell structure of this composite was characterized by SEM,TEM and FTIR.The nanosensor displayed strong emission intensity of Tb/GMP and linearly decrease with increasing concentration of O₂·-in the range of 0.5-6 ?M with as low as limit detection of 20 nM.And it exhibited good selectivity and high sensitivity in the HEPES buffer at pH 7.4.2.Dye@ metal organic frameworks in recent years has attracted more and more attention.In particular,it provides a good operating platform for us to build a ratiometric fluorescence analytical method.A facile one-pot stratege has been developed for synthesis of nanoscale ZnMOF74 encapsulating with fluorescein o-acrylate and Tris?2,2'-bipyridyl?-dichroruthenium?II?hexahydrate.The SEM?XRD were conducted for structure characterization.The results showed the complex exhibited good water dispersion.The nanosensor displayed strong emission intensity of F1 in 512 nm and Rubpy in 600 nm.The F512/F600 nm linearly decreased with increasing concentration of ClO-in the range of 0-100 ?M with as low as limit detection of 1.2 ?M.The composite materials showed well sensitivity for hypochlorous acid by ratiometric fluorescence method,which was expected to apply in quality testing of industrial goods and further biological cell experiments.3.A novel hybrid nano ratiometric fluorescence probe has been designed withsimply mixed carbon dots?CDs?and bovine serum albumin modified Au nanoparticals?BSA-Au NPs?.The nano hybrid probe exhibited tow emission peaks at424 nm for CDs and 640 nm for BSA-Au,respectively.Due to the strong coordination between Cu and S in BSA,the Au-S bond was destroyed resulting fluorescence quenching of BSA-Au.However,the addition of copper ions showed less influence on the fluorescence intensity of CDs.The ratio of F424/F640 nm linearly decreased with increasing concentration of copper ions in the range of 0-600 nM with the lowest detection limit of 0.5 nM.In addition,the interference of other metal ions is almost negligible,reflecting the ratiometric fluorescent probes is of high selectivity for copper ions.