| Fluorescence imaging recently has become one of the most influential techniques for tracing biomolecules in living systems due to high temporal and spatial resolution,safety and simplicity of implementation,while fluorescent probe with excellent performance is the foundation for imaging.In this paper,a novel rhodol dye ROA has been designed and prepared.At the same time,three kinds of fluorescence probes have been designed and researched by using ROA as signaling fluorophore.In detail,the thesis consists of three chapters as following:(1)Based the model platform ROA,a novel fluorescence probe RO610 was designed and synthesized for the efficient detection of ClO-.To the expected,the probe itself was nearly nonfluorescent due to C=N isomerization,while the strong yellow fluorescence was restored after addition of ClO-because of the formation of formyl group caused by oxidation and then hydrolysis of C=N.Furthermore,RO610 is capable of imaging exogenously ClO-in living cells and living mice.(2)A novel xanthene fluorescence probe has been designed by modifying the aldehyde with hydrazine group,which possesses both C=N and hydrazine group and is able to detect ClO-with high selectivity,excellent sensitivity and fast response.1H NMR titration and HRMS analysis were carried out to investigate the singnaling mechanism of probe with ClO-.The experimental results revealed that,after ClO-reacted with hydrazine group,a stable compound RON was formed by oxidation and then intramolecular proton transfer.This strategy also provides a new idea for the design of fluorescence probes for detecting ClO-.(3)A novel xanthene fluorescence probe RO587 has been designed and synthesized based on the novel fluorophore ROA.The fluorescence of RO587 was effiectively quenched due to the coordination to a paramagnetic Cu2+ center.The sensitive signaling is based on the efficient sequestering of complexed Cu2+ ions owing to the stable complex formation with sulfide ions,and could be used for the sensing of sulfides in complex system without interfering by common anions and sulfur-containing biological molecules.It is thus the contention that this finding would be a brand new guideline in designing reaction-based fluorescent turn-on sensors for the sulfides. |
