The Research Of Tenebrio Molitor L.Feeding On Lemna Minor L.

In this paper,the artificial feed of Tenebrio molitor L.was prepared from duckweed as the main raw material,and the biological chain relationship of duckweed and Tenebrio molitorL.was established.Through studying on various optimum biological conditions of Tenebrio molitorL.feeding on duckweed,the changes of intestinal bacterial composition and the changes of insect resource components of Tenebrio molitorL.,to provide a scientific basis for the treatment of duckweed by using Tenebrio molitorL.and to explore a biological system technology for the treatment of eutrophic organic wastewater.The results are as follows:1.Study on artificial feed of Tenebrio Molitor L..by comparing of different percentage of duckweed feeds on the larvae of Tenebrio molitor L.,it is found that:the group consists of"50%wheat bran:50%duckweed" makes the largest polypide increment rate,highest efficiency of feed utilization,feed conversion rate,whereas the lowest fatality rate.So,"50%wheat bran:50%duckweed" was the best feed.2.Effects of artificial diets with different water content on the larva of Tenebrio Molitor L..By comparing of the effect of different water content of duckweed feeds on the larvae,it showed that:when the water content of duckweed feed was 18%,the larvae have the largest polypide increment rate and the highest feed utilization rate,so,18%is the best humidity range of duckweed feed.3.Comparison of growth of the larvae with different densities under artificial feeding.By comparing of the biological indicators in different densities of mealworms fed with duckweed feeds,it is found that:when the larvae density was 0.56-1.12 heads per cubic centimeter,the growth rate of biomass of the larvae was the highest,at the range of 39.90%-39.73%.With the larvae density increasing,the growth rate of biomass of the larvae gradually decreased,when the larvae density reached 3.36 heads per cubic centimeter,the biomass of larval growth rate dropped to 4.25%.In addition,the feed utilization rate of the larvae showed the same trend with the rate of biomass growth.Therefore,1.12 heads per cubic centimeter was the best density range.4.By comparing the differences of the adult of mealworms fed with different feed additives,it is found that:compared to the bean cake,use duckweed as the feed additives of the adult can make its average life expectancy prolonged five days,single female fecundity increase 124 and the Spawning days increase about seven days.5.By comparing the resource composition of the two groups of larvae of mealworms which respectively fed on pure wheat bran and duckweed feed,in conclusion that,the larvae fed with duckweed feeds contains protein 61.7 0%,crude fat 9.7%,crude fiber 10.8%,ash 9.3%every hundred grams of dry matter;the larvae fed with pure wheat bran contains protein 52.6%,crude fat 29.9%,crude fiber 4.6%,ash 3.5%in the same weight.In addition,the larvae in two groups respectively have 17 kinds of amino acid.The ratio of essential amino acids and non-essential amino acid ratio(EAA/NEAA)was 69.21%of the larvae fed with duckweed,the pure wheat bran group was 69.25%.the ratio of essential amino acids and total amino acids,respectively,duckweed feed group 32.32%,pure wheat bran group was 40.90,both of them were around 40%.6.From the intestinal tract of pure wheat bran feeding larvae,two kinds of dominant bacterial strains were purified,belonging to genus Bacillus,Brevundimonas;and from the intestinal tract of duckweed feeding larvae,five dominant bacterial strains were purified:including the two kinds mentioned above and three more-Chryseobacterium Escherichia,Serratia.To provide a reference for further study on the internal mechanism of Tenebrio molitor L.using duckweed.