Studies On The Nutritional And Chemical Constituents Of Pholiota Nameko

This thesis consists of five chapters:the first chapter summarizes the research status of Pholiota higher fungi on chemical constituents and pharmacological activities;the second chapter content on the determination of nutritional components of Pholiota nameko were analyzed;the third chapter,chemical composition from fermentation liquid of Pholiota nameko and the anti-tumor activity of compounds were studied;in the fourth chapter,chemical composition of Pholiota nameko fruiting body was studied;the fifth chapter summarizes the research results.Pholiota nameko is a strophariaceae,Pholiota higher fungi,which is also known as Pholiota adiposa,and it is an artificial fungus.It can get the name because of its surface contains pectin.Its appearance is bright,taste delicious,nutrient rich,in addition to edible value,but also has a higher anti-tumor and other medicinal value.In the thesis,the nutritional components of Pholiota nameko were detected.As the meanwhile,the fermentation liquid and fruiting body of Pholiota nameko was studied.At the same time,anti tumor activity of some compounds was test.The nutritional compositions,trace elements and vitamin of Pholiota nameko were test,which include 17 kinds of amino acids,10 kinds of trace elements,7 kinds of nutritional composition and one kind of vitamin.The results show that it is rich in nutrients and has high edible value.We used a variety of means of purification,such as silica gel column chromatography,Rp-18,MPLC,Sephadex LH-20 and HPLC.l2 compounds were separated from the fermentation liquid of Pholiota nameko by modern spectroscopy technology(13C-NMR,1H-NMR,MS,HR-MS,IR),including 3 new compounds.The types of compounds are sesquiterpenes,sterols,derivatives of furan,adenosine.The antitumor activity of the separated sesquiterpene was studied.At the same time,4 sterols were separated from the fruiting body of Pholiota nameko by modern spectroscopy technology.