Research On Selectingand Incubating Excellent Yeast In Fermentation Characteristics From Fruit In Hohhot

The experiment research on the early stage of the group separation of 7 strains of yeast fermentation characteristics of comparative study,do experiments on seven strains of high quality yeasts for fermentation characteristics.The excellent strains with good fermentation characteristics were selected as the original strain for protoplast fusion and then produce strains with excellent traits.First test of the biochemical characteristics of the seven strains of yeasts were analyzed and the results showed that the fermentation characteristics of strain A and strain B were best.Be able to grow and multiply in a fermentation medium with the sugar concentration is 50%,a temperature is 43?,SO2 concentration is 350mg/L,pH is 2,and an alcohol is 16%(v/v).The protoplast formation and regeneration conditions of strain A and strain B were studied.The results showed that the preparation was carried out at 28 ? for 20 min and 0.2 g/L of snail enzyme at 37 ?.As the osmotic stabilizer,the protoplast formation rates of strain A and strain B were 98.11%and 97.24%,and the regeneration rates were 17.09%and 15.42%.The protoplasts of strain B,which was heat-inactivated for 40 min at 70 ?,and the protoplasts of strain A,which were inactivated for 120 days under UV,were fused to obtain 196 fusion element.Then we select 55 colonies with individual full,larger diameter and finally pick up 10 fusion fractions with better fermentation ability.With further re-screening and fusion sub-genetic stability test,strain R8 whose fusion effect was slightly better,total weight loss was 13.87g,residual sugar content of 13.7g/L,alcohol degree 5.1(v/v).The 268 rDNAD1/D2 sequence analysis was carried out to determine the strain R8 as Clavispora lusitaniae.