The Preparation And Biological Performance Of Titanate Nano-gridding

Titanium based biological materials as a traditional bone implant material,with high mechanical strength and fatigue-free property,have received much concern in the field of orthopaedic clinic.But the surface of titanium is bioinertia and without biological performance such as induction of bone formation ability.In order to enhance the osseointegration between Ti implant and native bone,physical chemistry are made on the Ti substrate surface,making the Ti-based implants as multifunctional bone implant material,rapidly integrating with native bone in vivo,enhance the use life of material.In this study,we fabricated alveolate double layer titanate nano-gridding structure based on Ti substrate,and researched the biological performance of the titanate material in vitro and in vivo.The specific contents are as follows:(1)Osteogenesis and angiogenesis have been considered to be the most direct and necessary requirements for Ti-based implants used in bone regeneration.In this study,an alveolate double layer SrTiO3 nano-gridding(NG-Sr)was obtained using two steps of anodization followed by a hydrothermal process.Different Sr gradient contents(NG-Sr0.02,NG-Sr0.04,NG-Sr0.08,NG-Sr0.12 and NG-Sr0.16)were generated from altering the precursor solution concentrations.The in vitro studies indicated that NG-Sr samples with nanoscale topography and long-lasting Sr release could enhance the biofunction of both osteoblast cells(MC3T3-E1 cells)and human umbilical vein endothelial cells(HUVECs)to varying degrees.The NG-Sr0.08 was the optimal substrate for MC3T3-E1 cell growth,while the NG-Sr0.04 was the best choice for HUVEC growth.In vivo x-ray analysis and histological observations demonstrated that the NG-Sr0.08 samples had notable osteogenesis and angiogenesis as significant new bone formation and fresh blood vessels were observed.Hence,the nanoscale topography with sufficient Sr supplementation offers a new independent regulator of two types of cell growth,which should be considered in the biomaterial design for regenerative medicine.(2)Fabricating alveolate double layer TiO2 nano-gridding based to Ti substrates through two steps of anodization,and then translating into Zinc titanate by hydrothermal process,finally,Ti-based implants with Zinc titanate nano-gridding coating were obtained.The effect of different concentration of Zinc on osteosarcoma cells(MG63)and osteoblast(MC3T3-E1)were explored by changing the precursor solution concentrations to generate different Zinc gradient contents(NG-Zn0.01,NG-Zn0.05,NG-Zn0.10,NG-Zn0.15 and NG-Zn0.20).The experiment results showed that the release of Zinc ions has obvious inhibiting effect on the growth of MG63 cells,and the inhibiting effect had positive correlation with Zinc ions concentration.The Zinc ions that release from the NG-Zn samples had no negative effect on the growth of MC3T3-E1 cells except NG-Zn0.20.It meant that,when the Zinc ions under a concentration,the NG-Zn sample can inhibit the growth of MG63 cells and ensure the normal growth of MC3T3-E1 cells at the same time,and it could be used as bone repair material after bone tumor resection.In summary,based on Ti substrates,double layer SrTiO3 nano-gridding and double layer Zinc titanate were fabricated through twice anodization and hydrothermal process.The results performed that SrTiO3 materials could promote the formation of new bone and stimulate angiogenesis,Zinc titanate materials were able to inhibit the growth of MG63 cell and maintained good biocompatibility.