The Osteogenesis And Antibiosis Performance Of Rifampicin/CuO-loaded Titanate Nano-gridding

Bone defect is the destruction of bone structure caused by debridement such as infection,tumor,trauma and various congenital diseases,which threatens human health to varying degrees.In order to improve the therapeutic effect and prevent secondary surgery,preparing a multifunctional bone implant material is essential.In this thesis,bone repair materials with both osteogenic and antibacterial functions were prepared by structural modification,coating modification and drug loading on the surface of titanium metal.The effects of materials on cell and bacterial growth were investigated and the osseointegration and antibacterial activity of the material were evaluated.The details are as follows:?1?In this study,the titanium dioxide?TiO₂?nano-gridding was prepared on the surface of Ti-based by two steps of anodization.Then the TiO₂ nano-gridding was transformed into the zinc titanate?ZnTiO₃?nano-gridding by hydrothermally treated.Subsequently,the hydroxyapatite coating and rifampin were modified on the surface ZnTiO₃.In vitro cell experiments showed that the prepared material had no obvious cytotoxicity,and the material coated with hydroxyapatite significantly promoted the growth and migration of osteoblasts?MC3T3-E1?.Antimicrobial experiments showed that materials containing Zn or rifampicin had certain antibacterial properties against Staphylococcus aureus?S.aureus?,and materials containing both Zn and rifampicin had the best antibacterial properties.The results showed that the Zn-NG-HA-RIF can simultaneously promote osseointegration and inhibit bacterial growth,and Zn and rifampicin have synergistic antibacterial effect.?2?In this study,a uniform TiO₂ nano-gridding film was prepared on the surface of Ti by a two-step anodic oxidation,and then conversion of TiO₂ nano-gridding to calcium titanate?CaTiO₃?through hydrothermally treated.The copper-containing CaTiO₃ nano-gridding?Cu/Ca-NG?was obtained through loading CuO on the surface of CaTiO₃ nano-gridding.The results of biological experiments showed that the CaTiO₃ nano-gridding array was beneficial to the adhesion and proliferation of MC3T3-E1 cells,and promoted the osseointegration.Cu-doped materials had a significant inhibitory effect on the growth of S.aureus.The results show that Cu/Ca-NG can effectively promote new bone formation and inhibit bacterial growth at the same time.In summary,we used pure metal titanium sheets as the base material to obtain two kinds of bifunctional bone defect repair materials through element doping,biomimetic coating preparation and drug loading,which can not only effectively promote new bone integration,but also effectively inhibit the growth of bacteria has potential value in the treatment of bone defect repair.