Self-assembly And Fluorescence Sensing Application Of Magnetic Particles

With the help of the self-assembly of hydroxyl iron oxide nanoparticles?FeOOH?,We by controlling the different conditions,preparation of different size of spherical hydroxyl iron oxide nanoparticles.Fluorescent probes are powerful tools for the investigation of biochemical processes,diagnosis of disease markers,detection of hazardous compounds,and other purposes,due to their high sensitivity,simplicity,as well as versatility.In virtue of the self-assembly of magnetic nanoparticles,by magnetic separation and subsequent plasmon enhanced fluorescence?PEF?,an assay platform with a signal output from completely ??zero?? background to fluorescence amplification is achieved,using quantum dots as reporters.So,it well breaks through the conventional ??turn-on?? strategy in both lower and upper limits.The sensitivity for hyaluronidase sensing is enhanced 104–106 times as compared with previous fluorescence methods.This article is divided into four chapters are listed as following:The first chapter is introduction of this thesis.First,the performance,basic property,common synthetic methods and applications progress of the FeOOH nanomaterials,semiconductor nanomaterials and magnetic nanomaterials was reviewed.At last,the existing problems and our working goal were put forward,respectively.In second chapter,by controlling the flow of time,pH and the amount of raw materials,we successfully prepared size-controllable monodispersed spherical FeOOH nanoparticles.In third chapter,in virtue of the three kinds of NPs?QDs,Fe₃O₄ NPs and Ag NPs?can be versatilely functionalized and bio-conjugated by sophisticated chemistry,we present a novel assay system with a unique signal output from completely zero background to genuine fluorescence amplification,enabled by a combination of magnetic separation and plasmon enhanced fluorescence.To demonstrate the proof-of-concept,hyaluronidase?HAase?,one of the hydrolases and cancer biomarkers,is assayed in both buffer solution and human biological fluids.The characteristic,innovation of this paper and the prospect of work are summarized in the fourth chapter.We first summarized the lightspot of the article,then the development direction of work is also prospected.