Research On The Extraction Of Phyto-salt From Suaeda Salsaand Its Polysaccharide Functional

Suaeda salsa was a kind of dominant halophyte which could preferably adapt to the saline and alkaline land. It is a kind of natural,non pollution and richly nutritious food resources which could be used to develop phyto-salt. It would have a higher application value. The paper intends to reasearch the extraction of phyto-salt and the functional constituents from Suaeda salsa. It could not only develop new varieties of functional edible salt,but also can effiectively use their own advantages of Suaeda salsa, which is proposed to provide the technical support for the development and utilization of phyto-salt.The mineral elements of Suaeda salsa in different growing period were tested, the contents of K, Na of its vegetative growth stage were highest whichrespectively were 1.17 g/100g, 5.21 g/100g. Box-Bchnken optimization design was conducted to the further research based on the results of single factor experiments of the extracting and decoloring of phyto-salt from Suaeda salsa process conditions . The optimum process conditions were determined: the solid to liquid ratio was 1:20 g/mL, extraction temperature was 93 ? and extraction time was 5 h, the experimental yield of water extract content was 50.23%; Under these condition, the best technologyical process solution of decolorization was that put 1.0% active carbons to phyto-salt solution at 50 ?,pH 5.5 with 10 minutes process time.The decolorization rate was 95.23%.The elementary composition of phyto-salt were the contents of K?Na respectively in 2.80 g/100g?15.62 g/100g, protein 11.53%, total sugar 13.13%, water 3.19%, ash 62.03%.As for volatile flavor components,39 varieties were found in the phyto-salt,which accounted for 96.67 in all, and the hygienic index was tested.Box-Bchnken optimization design was conducted to the further research based on the results of single factor experiments of the extracting of Suaeda salsa polysaccharide process conditions. The optimum process conditions were determined: the solid to liquid ratio was 1:15 g/mL, extraction time was 120 min, extraction temperature was 90 ? and extraction times was 3. Under this extraction condition, the yield of Suaeda salsa polysaccharide was 14.38%. Suaeda salsa polysaccharide was isolated and purified by Sephadex. The HPLC was used to analysis its molecular weight. The average molecular weight of Suaeda salsa polysaccharide were 2.52×106 Da. Some of the physicochemical properties was analysed. The results were total sugar 53.51%, reducing sugar 5.05%,polysaccharide 48.46%, uronic acid 12.93% and protein 1.09%. The Suaeda salsa polysaccharide solution was belong of bilge plastic fluid.In the first place, the vitro antioxidant activity was studied in biological activity. When the concentration of Suaeda salsa polysaccharide was 3.0 mg/mL, the clearence rates of Suaeda salsa polysaccharide towards DPPH,ABTS and hydroxyl radicals were 63.05%,76.15%, 53.12% respectively. Secondly, the antitumor effect of Suaeda salsa polysaccharide was analysed. The results showed that the inhibition rate of HepG2 cell were respectively 54.99%,72.72% in 24 h?48 h when Suaeda salsa polysaccharide was 12.8 mg/mL. The Suaeda salsa polysaccharide has good inhibitory effect on tumor cells.