The Construction And Application Of Tempo-oxidized Fe³⁺ Crosslinked Starch Nanoparticles Based On Light And PH Response

Malignant tumors are a major problem that threatens human's health.There are three main ways to treat cancer: chemotherapy, radiotherapy and surgical treatment. Drug chemotherapy is still the mainstream of the treatment, but there are many drawbacks in traditional drug chemotherapy,such as drug side effects, poor selectivity, drug dose and other shortcomings lead to low cure rate of the tumor, the patient treatment process to bear a variety of complications In view of the above problems,drug carrier targeted delivery therapy becomes significant, it not only improves drug effects, but also reduce the side effects, reduce the various complications caused by tumor therapy.To achieve the goal for the above-mentioned, we constructed a highly responsive oxidized starch nanosparticles based on pH and light response according to the specific microenvironment of the tumor. First,intravenous injection into the blood, through the pH response specifically targeted to the tumor site and a large number of enrichment; Followed by the cells into the near-infrared light 980 nm after irradiation response to the release and release of anti-cancer drug Dox, to achieve high efficiency anti-tumor effect.The paper mainly includes the following three aspects:1. Preparation of uniform, stable iron-crosslinked starch nanoparticles with uniform particle size. The nano-microspheres were prepared by membrane emulsification and ultrasonic cutting. The nano-microspheres with stable grain size at about 200 nm were obtained.2. Construction and characterization of light response drug carrier.The modified nano-microspheres were modified with PEI and DMMA,embedded Dox, and characterized by modification and embedding. The experimental results show that the photo-responsive carrier can resuit the charge-overturn at pH 6.8 in vitro, and can release Dox at 980 nm near infrared light.3. Responsive characterization of photoperiodic response vector. The prepared vectors exhibited good responsiveness in vitro. Cytotoxic assays of different components, and cell uptake experiments by laser confocal and flow cytometry also showed good dual responsiveness of the vector.Further in vivo biopsy experiments in mice again validated the good performance of starch nano-spheres based on light response and pH response.