Development On The Detection Method Of Glycidyl Esters In Edible Oils

Because of the potential health risks, fatty acid esters of chloro propanediol(MCPD-Es)and glycidol (Gly-Es) in foods are drawing the attention of Public Health Authorities. Today it is known that free form 3-chloro-1,2-propanediol (3-MCPD) had been known as contaminants in acid-hydrolyzed vegetable protein, and free form glycidol (Gly)had been classified as Group 2A probable human carcinogen by IARC. Fatty acid esters of 3-MCPD (3-MCPD-Es) and Gly (Gly-Es) have been reported to occur during purification and deodorization of fats and oils. The concerns about their potential health risks have prompted development of methods for quantitative determinations of these compounds.The purpose of this study is building a new and rapid method used to detect the content of fatty acid esters of glycidol in edible oils. Firstly, after acid catalytic derivatization with Levis acid and 2,4-DNPH, the quantitation of fatty acid esters of glycidol was achieved by using UV-VIS spectrophotometer and high performance liquid chromatography, and the calibration curves of different fatty acid esters of glycidol were made, the R2 of glycidyl esters, palmitate, oleate and linoleate glycidyl 'esters were 0.9948,0.9920, 0.9986 and 0.9990 respectively. Finally, the concentration ranges detected in edible oils were 0.99-15.70 mg/kg for glycidyl esters, 1.42-4.82 mg/kg for glycidyl palmitate and 0.58-6.82 mg/kg for glycidyl oleate, the relative standard deviation were 1.7-7.2%,4.2-9.2% and 4.1-17.4%, respectively. Moreover, the limit of detection for glycidyl esters,palmitate and linoleate glycidyl esters were 0.80 mg/kg, 0.18 mg/kg, 0.10 mg/kg and 0.09 mg/kg, respectively. When glycidyl ester of palmitic acid was added to the oil samples, the recovery rate was calculated to be 96-108%.