Screen Of Lactic Acid Bacteria With Antioxidant Activity From Fermented Meat Product And Studing On The Antioxidant

In this experiment,according to the screening criteria of meat fermentation agent,30 lactic acid bacteria were isolated from 6 fermented meat products.The antioxidative effect and some influencing factor were studed.Meanwhile,the highest antioxidative strain was identified by carbohydrate fermentation,physiological and biochemical tests and 16SrDNA sequence analysis.In order to further confirming antioxidative effect,we use the highest antioxidative strain to feed mice which were given subcutaneous injection of D-gal continuously mice,verify antioxidative effect in vivo.By the antioxidant activity of fermentation supernatant,results show X3 1 had the strongest scavenging ability of DPPH free radical(62.17%);the strongest scavenging ability of superoxide anion(22.96%);the strongest chelate iron capacity(93.02%);the strongest reducting ability(0.78)and the strongest resistance to lipid peroxidation(52.45%).While X31 had higher hydroxyl radical scavenging activity than X31(P<0.05).So X31 demonstrated the better potent activity on antioxidant capacity than others.The strain wsa identified as Pediococcus Pentosaceus by carbohydrate fermentation,physiological and biochemical tests and 16S rDNA sequence analysis.According to the experiment,we found that 6 of pH,37 ? and the 18h of culture time,strains had the highest antioxidant activity.Higher antioxidant activity of lactic acid bacteria in vivo was studied.The activity of antioxidase system enzymes,SOD,GSH-PX,CAT,T-AOC and MDA in mice liver,kidney and serum were tested.The TG and T-AOC of serum were also tested.It is seemed that the VC and X31 can increased the SOD,GSH-Px,CAT and T-AOC activity and decreased MDA,TG and T-CHO concents of mice.So X31 can improve the antioxidant ability of mice,and thus to protect the body.