Synthesis And Properties Of Multi-responsive Drug Carriers Based On Functionalized Nanoparticles

As the third largest diseases, cancer has been a serious threat to human health. Currently,effective clinical treatment of cancer is generally conventional chemotherapy. Chemotherapy is the use of anticancer drugs to inhibit rapidly dividing cancer cells. However, the chemotherapy drugs without targeting kill cancer cells while normal cells tissue will be destroyed. Thus the application of chemotherapy drugs will be limited. Combined treatment of drug carriers with chemotherapy drugs provides a new direction for cancer treatment, but the size, shape, structure, surface functional groups, and other parameters of carriers have limited their practical application. Therefore, the design of new multifunctional ideal drug carriers is one of the main research directions in the drug carrier field.Three kinds of multifunctional magnetic nanocomposite have been prepared in this paper.We investigated the feasibility of nanomaterial as a drug carrier by associated characterization and performance tests. With magnetic nanomaterial as the core, the core-shell structure magnetic nanocomposites with multiple responses were prepared by solvothermal,layer-by-layer electrostatic self-assembly and in situ synthesis methods. Meanwhile, the preliminary studies of composite were carried out including the morphology, structure,responsiveness and the performance in the drug delivery field, as follows:Fe3O4-CMC, a Carboxymethyl cellulose functionalized magnetic nanomaterial, has been successfully synthesized using a simple solvothermal. The synthesis method of nanomaterials is simple and the product shows a spherical structure with good dispersibility. Moreover, the average particle size is consistent with the needs of ideal nanocarriers. Specially, the introduction of carboxymethyl cellulose offers rich carboxyl functional groups with a large number of the active site which is beneficial to load and release the drug molecules. Therefore,we focused on the influence of carboxymethyl cellulose on nanomaterial, biocompatibility and drug release properties of pH response.Subsequently, Fe3O4-CMC@Zn-PA, a core-shell structure magnetic nanocomposite with double-responsive, has been designed using Fe3O4-CMC as substrate. Nanomaterials remain the spherical structure of the substrate and the size distribution is uniform. To assess the potential application of the composite as a drug carrier, drug loading and release properties were detected. In contrast to pure substrate, drug loading capacity of Fe3O4-CMC@Zn-PA has improved significantly. Moreover, the sustained release performance results clearly show that the carriers displayed properties of pH response. Meanwhile,due to the raw materials is the phytic acid which has significant anti-cancer effect, we focused on the corresponding cell cytotoxicity of the composite by MTT test to further validate the combined therapeutic effect of Fe3O4-CMC@Zn-PA.Finally, Fe3O4-CMC@ZIF-8@CDs, a core-shell structure composite with three-responsive, has been prepared using Fe3O4-CMC as substrate. The composite keep uniform spherical structure and the size increase significantly,but the size is still consistent with the ideal size of the drug carriers. The saturation magnetization of nanomaterial is 35.1 emu g-1 and fluorescent effects also achieve a significant improvement. In addition,the nanomaterial exhibits a high surface area, and drug loading capacity of nanocomposite gained greatly improvement compared to previous two nanomaterials. The sustained-release performance results also show that the nanomaterial has obvious properties of pH response.Due to its special structural design, we further verified the pH-responsive principle of Fe3O4-CMC@ZIF-8@CDs.