Study On The Synthesis Of Nanoparticles Coated By Phytic Acid And The Inhibition To MG-63

Current cancer chemotherapies face long-standing challenging obstacles.Conventional therapy,direct administration of therapeutic regimes to patients,is inclined to be eliminated instinctly by low cell-membrane permeability,poor physiological stability and nonspecific targeting,resulting in severe adverse effects on normal tissues.Nanoscaled carriers have emerged as promising candidates to solve the problems mentioned above in biomedical field,which is limited by the morphology,functional groups of the surface of the carrier and the low treatment effect and other factors.Thus,to explore novel multi-functional nanomaterials as drug delivery vehicles have been a hot issue in nowadays.Here three kinds of functionalized multi-response core-shell structure magnetic nanocomposites were prepared by solvothermal synthesis,co-precipitation and layer by layer electrostatic self-assembly method.We have discussed the feasibility of as-prepared products as drug delivery vehicles which may combine with anticancer drugs to inhibit the regeneration of cancer cell through characterization and performance test.The results are as follows.The base material Fe₃O₄ with narrow size distribution and good dispersity were successfully prepared by solvothermal synthesis.After that,Fe₃O₄ was functionalized by phytic acid?PA?by layer by layer electrostatic self-assembly to produce magnetic core-shell nanostructure carriers named Fe₃O₄@PA.In this procedure,PA was considered as surfactant,which offered rich phosphate groups and active sites on the material surface for drug loading,making the drug carrier pH-responsive in different pH conditions.Meanwhile,PA can be an active constituent combined with anti-cancer drugs to inhibit tumor cells regeneration.A novel magnetic nanocarrier Fe₃O₄-IP6 was synthesized by one-step co-precipitation using sodium phytate?IP6?as template.The obtained products with spherical structure showed uniform particle size distribution and good dispersity.In the above process,IP6 as auxiliary reagent successfully modified Fe₃O₄ magnetic nanoparticles,providing rich phosphate groups and large amounts of active sites on the material surface for drug loading,thereby improving drug loading efficiency to some extent.The results of drug release test in vitro demonstrated that as-synthesized carriers had obvious pH-response.In addition,IP6 itself had obvious anti-cancer effect so that we focused on detecting the cell cytotoxicity of nanomaterial and treatment effects of the carrier combining with anti-cancer drugs,which was verified by MTT test in the MG-63 cancer cell line in vitro.Magnetic basement Fe₃O₄@CS was fabricated by Introducing chitosan in one-step solvothermal synthesis,showing spherical structure with narrow size distribution and good dispersity.After that,Fe₃O₄@CS was functionalized by phytic acid?PA?by layer by layer electrostatic self-assembly to produce magnetic core-shell nanostructure carriers named Fe₃O₄@CS@PA.In this procedure,CS and PA were separately considered as surfactant and Structure stabilizer,which provided rich functional group and plenty of active sites on the material surface for drug loading,improving drug loading efficiency effectively.The influences of the introduction of CS and PA on drug loading efficiency and the pH-responsive release propertiy under different pH conditions were studied.Moreover,the other keystone was to discuss the propertiy of PA combined with anticancer drugs to inhibit the regeneration of cancer cells.