High Effective Adsorption,Separation And Detection Of Environmental Hormone Bisphenol A By Surface Imprinting Technique

Bisphenol A as a kind of environmental estrogen is harmful to human beings and other living things.When bisphenol A comes into the living body,which will interfere with the normal synthesis of endocrine,resulting in the body of the disorder of the major systems,a variety of diseases endanger health,and even affect human reproduction.Therefore,separation and detection of bisphenol A is of great significance.In recent years,solid phase microextraction in the tube for the separation and detection of trace bisphenol A has been vigorous development due to its advantages of low cost,automation and environmental friendliness.However,these extraction media have no specific selectivity to bisphenol A and are susceptible to matrix or co-occurrence interference,which affect the accuracy and sensitivity of the analysis.The polymer prepared by surface molecularly imprinted polymer has certain selectivity to the target molecule and has been widely used in the separation and detection of bisphenol A.However,some nonspecific adsorption on surface molecularly imprinted polymers effect on the separation efficiency.Aptamers have been widely used as a highly specific recognition material in the specific detection of bisphenol A.The paper studies the solid phase microextraction,surface molecular imprinting technique and the high specificity of the aptamer about high efficient separation and detection of bisphenol A,which provided a certain reference value for the separation and determination of bisphenol A.The specific research contents are as follows:1.In this chapter,the surface molecularly imprinted polymers(MIPs)for the specific recognition of bisphenol A were prepared by aptamer-affinity bisphenol A-oriented surface imprinting.The comparition the adsorption properties and selectivity about aptamer surface molecularly imprinted polymer microspheres and ordinary surface molecularly imprinted polymer microspheres showed that the aptamer surface molecularly imprinted polymer microspheres can increase the utilization ratio of template and form a more intensive hydrogen bond site.Hence,aptamer surface molecular imprinting leaded the adsorption capacity was two times that of ordinary surface molecular imprinting and high selectivity for the target molecules because of aptamer in imprinted cavities.This suggested that the aptamer modified surface molecularly imprinted polymer was promising to be a good candidate for selective extraction materials.2.In this chapter,a double-identified resonance light sensor based on the synergistic effect of the aptamers and surface molecular imprints was prepared for the detection of bisphenol A.In the study the effects of various conditions(such as pH,time,particle dosage,temperature,etc.)on the response light response and the selectivity and relative standard deviation of the target were researched.This kind of molecular imprinted resonance light sensor,which using nano-silicon material to cross-linked aptamer,has a certain reference value for the detection of bisphenol A due to the simple and economical preparation process.3.In this chapter,the surface molecularly imprinted polymer membranes,which has the advantages of controllable structure and thickness,high specificity and selectivity,were prepared by electro-polymerization on suitable tubular material using bisphenol A as template molecule.The effects of electro-polymerization various factors on the structure and properties of the surface molecularly imprinted polymers were studied.The method makes a good bedding for combining the surface molecularly imprinted tube with high performance liquid chromatography and establishing a system for analysis of solid phase microextraction coupled with high performance liquid chromatography,so as to establish on line selective separation and enrichment of BPA in environmental water samples.