A Study On The Catalytic Activity Of Iron Porphyrin And Its Application

Heme,a ferroprotoporphyrin,is an important part of hemoglobin which consists of four pyrrole subunits.So far,it was confirmed that hemoglobin which has good antioxidant activity could catalyze the reaction of tyrosine and hydrogen peroxide forming dityrosine and this function could protect HUV cell from the damage of ischemia reperfusion.Based on its nice antioxidant activity,we further verify and learn the effectiveness of protection from heme and tyrosine on HaCaT immortalizedhuman epidermal cells,reflecting its value in the application of anti-aging cosmetics.Two oxidative damage models were established to simulate the oxidative damage of skin cells.They are the glucose oxidase damage model and the UVA damage model,respectively.Under this condition,the protective effects of heme and tyrosine on HaCaT immortalized epidermal cells were explored.Study verifies the effect by 6 methods as follow:MTT method,LDH Cytotoxicity Assay,Lipid Peroxidation MDA Assay,Apoptosis Detection,Cell Cycle and Apoptosis Analysis,Western Blot.The antioxidant effects of well-known Vitamin C(50 ?M),Vitamin E(50 ?M)and BHT(butylated hydroxytoluene,50 ?M)were compared with heme and tyrosine.We found that the antioxidant effect of heme(50 ?M)with tyrosine(200 ?M)is better than either of them and even better than Vitamin C,Vitamin E and BHT.Experimental results show that heme itself has the nature of antioxidant,and in the presence of tyrosine,on the one hand,heme display its own antioxidant effect,on the other hand,it acted as a catalytic to catalyze forming dityrosine in the oxidation conditions.In general,heme with tyrosine has a synergistic protective effect under oxidizing conditions.It provided a theory for following study.