Study Of Optical Properties And Bioimaging Applications Of Au Nanorods Assembly

Non-spherical noble metal nanoparticles have more abundant,adjustable surface plasmon resonance behavior,good biocompatibility,low toxicity,and abundant surface modification,they are widely used in various bioimging applications.In this proposed parper,we synthesized gold nanorods and studied its optical properties and its application in bioimaging.This parper has four chapters as the following:The first chapter is the introduction.we first describled non-spherical noble metal nanoparticles and the basic properties of gold nanorods,especially the surface plasmon resonance properties.Then,we summarized some common synthetic methods and introduced the abundant surface modification and application as bioimaging sensor of gold nanorods.And put forward the problems to be resolved and the potential application,at last,put forward our working goal in this paper.The second chapter studies the factors that influence the gold nanorods surface modification with silicon dioxide layer in a series of different conditions,investigate temperature,pH,time,concentration of(TEOS)tetraethyl orthosilicate and cetyltrimethyl ammonium bromide(CTAB)accurately.Then we adjusted the thickness of silicon dioxide layer in the surface of gold nanorods,and then modified amino on the surface of silicon dioxide layer.Through the accurate control of experimental conditions,it can improve the repeatability of the experiment.The third chapter is firstly put forward for increase fluorescence of gold nanoclusters through the plasmon resonance properties of gold nanorods the first time,and increase its application in biological imaging.The gold nanoclusters was synthesized by bovine serum albumin(BSA)which have good fluorescence properties and biocompatibility,but itsquantum yield is low,generally between 4% and 7%,and limits its wider application.We have designed to improve the fluorescence of gold nanoclusters with the surface plasmon resonance properties of gold nanorods,and through regulating the distance between the gold nanorods and gold nanoclusters to examine the impact on the gold nanoclusters fluorescence.The Experimental results show that when the distance between the gold nanorods and gold nanoclusters is about 15 nm(the distance was controlled by the the thickness of silicon dioxide layer),the fluorescence intensity gold nanoclusters was maximum enhanced,is about 5 times;The lifetime of fluorescence was shorter than pure gold nanoclusters,.And because the gold nanorods — gold nanoclusters assembly surface has a lot of BSA group,so it don't need to further modify to enter cells,finally realize its dual function imaging in intracellular imaging with the dark field imaging and fluorescent applications.The fourth chapter for the innovation of this paper,characteristics and job prospect.We have made some summary of work,and the follow-up research work is prospected.