| With the rapid development of China's economy and the general improvement of people's living standards,the amount of food waste production is also increasing rapidly,many large cities daily production of garbage in more than 4000 t,of which about 50% of food waste[1].The processing of food waste is the world's countries are generally concerned about and urgent problems to be solved.At present,there are many ways to deal with food waste,both have their advantages and disadvantages.In recent years,there has been a study of the use of maggot insects bio-converted food waste,the use of insects to deal with food waste can not only be rich in protein and fat insect biomass and organic fertilizer,and high conversion rate,with no pollution,also intestinal microbes in the transformation of insects using food in the process has an irreplaceable role.However,there are few studies on the intestinal microbes of the Chrysomya megacephala larvae,which have a direct impact on bio-transformation of food waste,and little is known about the molecular mechanism of intestinal microbes in the process of biofilter.Therefore,in this study,we feed the different nutrient contents to the Chrysomya megacephala larvae for its passage,studied the effects of different nutrient contents on its intestinal microbes,and microbial molecular mechanism of intestinal microbes in the process of bio-converted food waste.Due to different sources of food waste nutrition is very unstable,former study found that the bulk of the Chrysomya megacephala larvae on the oil and protein content of different food waste has a significantly different bio-transformation efficiency[2].Therefore,we use artificial feed(60% bran,30% fish meal,10% blood meal)as a basic feed to simulate food waste,by adding soy oil and peptone to adjust the feed fat and protein content,the use of oil and protein content of the five kinds of different types of food waste to feed the bulk of the Chrysomya megacephala.On the one hand,the effects of different nutrient contents on their biological shape during their passage were analyzed,and their use of feed;on the other hand,16 S r DNA of the larvae intestinal microbes were sequenced to study the variation of intestinal microbial population diversity under the condition of changes of fat and protein content in feed,preliminary study on the mechanism of microbialmolecular ecology of bio-transformed food waste use the Chrysomya megacephala larvae.The main results of the paper are as follows:First,with the increase of fat content or protein content in the feed,the larval development period was extended to varying degrees.Compared with the control group,the development period of the high fat group larvae was 3.5 days longer than that of the control group.The high protein group larvae had an average extension period of 1.75 days compared with the control group.It is worth noting that the eclosion rate of the high protein group is 24% higher than that of the control group,indicating that the appropriate increase in the protein content in the feed contributes to the emergence of the bulk of the eclosion of Chrysomya megacephala.Second,with the increase of fat content or protein content in the feed,the weight of the high fat group A and the high protein group B were less than that of the control group.In the high fat group A,the weight was decreased by 2.6 times compared with the control group,and the group B was 1.85 times lower than that in control group.The conversion coefficient of group A was 1.79 times lower than that of control group.The fat and oil conversion coefficient of group B was lower than that of CK group,and the conversion coefficient of oil was 2.78 times lower than that of control group.The results showed that the oil content and protein content of the experiment did not affect its passage,but all had an effect on the growth of the Chrysomya megacephala,and the ability of the larvae to use the oil was higher than that of the protein,but the high fat diet easily led to the Chrysomya megacephala larvae die.Third,With the increase of the content of fat in the feed,the activity of esterase in the intestine of the Chrysomya megacephala larvae was increased.With the increase of the protein content in the feed,the trypsinase activity of the high fat group and the high fat group showed a decreasing trend,While the activity of valine aromatase showed an increasing trend.Indicating that esterase may be related to the use of oil and larvae,and high protein feed will inhibit the larval intestinal trypsinase activity,valine may be with the Chrysomya megacephala larvae of the use of feed protein.Fourth,With the increase of fat content in feed,the abundance and diversity of intestinal microbes increased first and then decreased.With the increase of protein content in feed,the microbial abundance of larvae The results showed that Wohlfahrtiimonas showed a tendency to increase first and then decrease,whether itincreased the protein content in the feed or the content of oil and fat.It is suggested that the increase of the content of the fat in the feed can increase the diversity of the intestinal microbes in the larvae,and the growth of the microorganisms in the larvae becomes unstable with the increase of the protein content in the feed.The results showed that the relationship between Wohlfahrtiimonas and Chrysomya megacephala larvae was needed to be further studied. |
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