The Relationship Between The Structure Of Intestinal Microbiota And Fecal Fatty Acids With Different Diet Patterns

The research on the influence of intestinal microbiota on human health was interested by scientists.The structure of intestinal microflora was affected by many factors.Researches showed that the environmental changes was the most important factor in intestinal microecology except genes in recently.How dietary effect on the intestinal microecology attracted more attention.The changes of intestinal microflora were affected by the type of diet and the composition of nutrients and some of them was the triggers of intestinal disease.Therefore,it is necessary to explore the relationship between different protein sources of diet patterns and the intestinal microflora and metabolites in ChineseFrom 2016,10 to 2017,3,we collected 16 feces samples from Qinghai province,Zhe Jiang province and Hei Longjiang province.Feecal samples were classified with different protein forms of diet patterns: The plant-rich protein sources diet consumption was vegetarian group?n=6?,animal protein sources diet consumption was omnivore group?n=5?and mix protein sources of diet consumption was control group?n=5?.The volunteers of each group kept their diet patterns for more than ten years.We tested the samples in two parts: fecal microbiota of richness and diversity was quantified using Illumina Miseq high-throughput sequencing,and the concentrations of lactic acid and short chain fatty acids?acetic acid,propionic acid,butyric acid,and isobutyric acid?of fecal were used high performance liquid chromatography technique.The relationship between fecal fatty acid and intestinal flora was explored by Spearman correlation analysis.The results showed that the diversity and richness of intestinal microbiota with different protein sources of diet patterns have changed.The ACE and Chao1 index increased significantly in vegetarian group?V group?and omnivore group?O group??P < 0.05?.The increase in Shannon diversity index was only observed in V group?P < 0.05?.The differences of bacterica in each level were observed: At phylum level,a significant increase abundance of Tenericutes in the V group and O group,and a decrease the of Cyanobacteria in O group.LESfe analysis showed the Enterobacteriaceae in V group increased.At the genus level,Ruminococcaceae_UCG₀14 and Christensenellaceae_R7group in O group?P < 0.05?,Ruminococcaceae_NK4A214_group?P < 0.05?and Prevotella₇?P < 0.05?in V group increased,Lachnospira decreased in both V group and O group.In Beta diversity analysis,PCA and UPGMA clustering results showed the samples with different diet patterns clustered,which demonstrated the structure of the intestinal flora that kept same diet of samples tends to be similarly.The concentrations of acetic acid?mmol/kg?in the feces of the O group was significantly increased.The concentrations of total short chain fatty acids in V group increased,but the increase was not significant.In addition,the differences of the concentrations of propionic acid,butyric acid and isobutyric acid were not significant among three groups?P < 0.05?.Spearman correlation analysis showed that Ruminococcaceae_NK4A214_group?P < 0.01?and Christensenellaceae_R.7_group?P < 0.01?were significantly positively correlated with the production of acetic acid,and Bifidobacterium and butyric acid were significantly negatively correlated?P < 0.05?.The results indicated the different protein sources of diet patterns have effected on the structure of intestinal microflora.The diversity and richness of intestinal microbiota have changed and the significant different species were observed at different levels.Fecal samples were clustered separately in beta analysis.In addition,the concentrations of fecal fatty acids have changed significantly.Dietary may have extent contribution to the changes of structure and activity of intestinal microflora.Our research enriched the status of the relationship between the intestinal microflora and diet patterns in Chinese.