Synthesis Of Functionalized Magnetic Microspheres For Selective Recognition And Separation Of Hemoglobin

It is well known that the successful proteomics research mainly depends on innovation and progress in the large-scale high-throughput separation and analysis technology.In recent years,the researchers have reported different types of functionalized magnetic microspheres for the separation of proteins through specific interaction(electrostatic adsorption and affinity)between protein tag and surface function ligands on the magnetic microspheres.In this paper,the two functionalized magnetic microspheres were synthesized through crosslinking reaction and distillation-precipitation polymerization,and successfully applied to the separation of hemoglobin.1.Fe3O4 magnetic microspheres were synthesized through a modified solvothermal reaction and then were conveniently surface-coated with silica layer through a sol-gel approach to prepare core/shell structure Fe3O4@SiO2.The structure was modified with 3-aminopropyltrimethoxysilane(APTES)as anchor molecules to donate aminopropyl group via the silylation reaction.Finally,the aminopropyl group on the surface of magnetic microspheres reacted with 2-aminothiazol(AT)to synthesize Fe3O4@SiO2@AT through the glutaraldehyde as crosslinking agent by nucleophilic substitution and then dehydration reaction between amino groups and aldehyde groups to form imine.The adsorption experimental results showed that Fe3O4@SiO2@AT exhibited high hemoglobin binding capacity,high efficiency and excellent selectivity in the isolation of hemoglobin.2.Fe3O4 were synthesized through a modified solvothermal reaction and then were conveniently surface-coated with silica layer through a sol-gel approach to prepare core/shell structure Fe3O4@SiO2.Fe3O4@SiO2@MPS was prepared via the silylation reaction with 3-(methacryloyloxy)propyltrimethoxylsilane(MPS)as anchor molecules to introduce active vinyl groups.High density poly(4-vinylbenzylchloride)(pVBC)shell was modified on the surface of Fe3O4@SiO2@MPS via distillation-precipitation polymerization Fe3O4@SiO2@pVBC.Finally,the chemically bonded chloromethylbenyl group was reacted with N-methylimidazole to obtain the imidazolium ionic liquid modified magnetic microspheres(Fe3O4@SiO2@pVBC@IL).The results of isothermal,selective and dynamics adsorption experiments showed that Fe3O4@SiO2@pVBC@IL had a high capacity,good selectivity and quick balance of hemoglobin adsorption.At the same time,the functionalized magnetic material was used to separate hemoglobin from the human whole blood,which proved that the synthesis of magnetic composite microspheres could be an ideal material for the isolation of hemoglobin from the complex samples.