Study On Extraction Of Tomato Seed Oil By Aqueous Enzymatic Method And Demulsification

Tomato is the local characteristics of Xinjiang vegetable crops,a byproduct produced during processin g of tomato seeds contained about 15%~30% crude fat,in order to improve comprehensive utilization value of tomato in this experiment,tomato seed oil extraction technology conditions of raw material,tomato see d discusses using water enzyme method,and get rid of the emulsion to produce the enzymes in the process of solution,and to study the physicochemical properties of tomato seed oil,the content of unsaturated fatty acids and volatile aroma components.Object:This paper aims to extract the tomato seed oil and its basic process and its parameters are optim ized by using a method of safety green pollution-free water enzyme method;the best demulsification proce ss was determined through analysis and comparison of different demulsification process,to further improve the oil extraction rate;to evaluate the quality standard of water enzyme in tomato seed oil method and prod uction,and its volatile aroma components were determined,in order to provide theoretical and practical bas is for the high value utilization of tomato seed oil.Methods:Based on the reference and the pre experiment,this study selected three acid protease,cellula se and pectinase enzymes were optimized,and the effects of enzyme dosage,pH,material liquid ratio,enzy molysis time and enzymolysis temperature and other factors affecting the oil extraction rate of tomato seed oil,and the surface of it obtained the optimum enzymolysis response of process conditions on the basis of single factor;based on the above research,the research on the emulsion produced during the enzymatic hyd rolysis of the enzymatic demulsification,regulating pH demulsification,ethanol demulsification,inorganic salt,freezing-thawing demulsification demulsification,centrifugal demulsification of 6 commonly used de mulsification methods comparing,and determine the best demulsification method and the process paramete rs on tomato seed oil;physicochemical indexes,fatty acid composition and volatile aroma components anal ysis.Results and Conclusion:(1)Determined by acid protease,pectinase and cellulase enzyme three com posite enzymatic extraction of tomato seed oil,response surface optimization of the experimental condition s by using Design-Expert on the basis of single factor,the optimal process conditions and parameters wer e: enzyme dosage 2.5%,enzymolysis temperature is 45 ?,enzymolysis time is 2.5 h,solid-liquid ratio wa s 1:5(g/mL),the theory of predictive value of 43.362%,after verification of tomato seed oil extraction rat e was 43.244%,and the theory is close to the predicted value,which shows that the model has high reliabili ty,can well predict the test results.(2)The experiment found that freezing and thawing was(the temperature is-20 DEG C when the oil e xtraction rate is 56.98% pH(pH);extreme 11 demulsification of oil extraction rate reached 61.99%);ethan ol assisted demulsification(ethanol concentration of 20% oil extraction rate was 59.12%);inorganic salt(so dium chloride assisted demulsification of oil demulsification the rate was 52%,the extraction rate is 51.99% sodium chloride);enzymatic demulsification of oil extraction rate was 51.57%,the centrifugal demulsifi cation of the oil extraction rate was 56.98%,compared the three good demulsification effect,combined with the actual production,determine the best demulsification process and its parameters were: ethanol concen tration 20%,the oil rate is 59.12%(3)Water enzyme preparation tomato seed oil physicochemical indexes are as follows: the total content of unsaturated fatty acids was 80.1%,relative density of 0.9187,moisture and volatile matter 0.01%,iodin e value is 119 gI/100 g,saponification value 202.7 mgKOH/g,acid value is 0.51 mg/g,the peroxide value is4 mmol/kg,compared with the hygienic standard of enterprises the results showed that the nutritional inde x of tomato seed oil,aqueous enzymatic extraction technology of tomato seed oil conforms to the enterprise standard of tomato seed oil,oleic acid and linoleic acid(21.7%)and(58%)content that aqueous enzymatic extraction of oil well preserved fat nutrition.(4)A total of 72 volatile components were detected from the tomato seed oil by headspace solid phase microextraction and gas chromatography-mass spectrometry(GC / MS),including alkane(8),olefinic(5),aldehyde(19),ketone(8),alcohol(15),aci(5),ester(3),heterocyclic(7)and others(2).The total content of volatile compounds in aldehydes,ketones,alcohols were relatively high,followed by 44.46%,13.25%,10.93% respectively,to provide the basis for quality control and rapid detection of tomato seed oil.