Study On The Amplication By Gold Nanoparticles Based On Capillary Electrophoresis

This paper mainly studied the nano materials in the application of capillary electrophoresis online enrichment methods,because the nanometer material has good biocompatibility,large specific surface area,stable physical and chemical properties,it has showed significant effect on the capillary online enrichment methods.Through the application of nanometer materials,the effect of enrichment is significantly improved.This article is divided into three chapters:Chapter 1,The main technology of capillary electrophoresis are introduced.First,the development and application of capillary electrophoresis are discussed,focusing on several separation modes of capillary electrophoresis;second,the development of capillary electrophoresis on-line enrichment methods were summarized;third,looking forward to the capillary electrophoresis research status both home and abroad,and introduced some innovation which plays an important role in capillary electrophoresis development;finally,introduces the application of nanometer materials in the application of capillary electrophoresis,and enumerates several important nanomaterials which possess some functional characteristics and all of them play a important role in capillary electrophoresis separation methods.Chapter 2,ultrasensitive and accelerated detection of CTX3 C by capillary electrophoresis via on-line sandwich immunoassay with rotating magnetic field and nano particles signal enhancement.A sensitive and rapid on-line immunoassay for the determination of ciguatoxin CTX3 C was developed based on a capillary mixing system,which was integrated with capillary electrophoresis(CE)separation and electrochemical(EC)detection.In the sandwich immunoassay system,anti-CTX3C-functionalized magnetic nanoparticles were used as immunosensing probes,and horseradish peroxidase(HRP)and anti-CTX3 C antibody were bound onto the surface of gold nanoparticles(AuNPs)and used as recognition elements.Online formation of immunocomplex was realized in capillary inlet end with an external rotating magnetic field.Compared with classical HPLC-MS and ELISA,the assay adopting AuNPs as multienzyme carriers and online sandwich immunoassay format with rotating magnetic field exhibited higher sensitivity and shorter assay time.The linear range of the assay for CTX3 C was from 0.6 to 150 ng·L-1 with a correlation coefficient of 0.9948(n = 2),and the detection limit(S/N =3)was 0.09 ng·L-1.The developed assay showed satisfying reproducibility and stability,and it was successfully applied for the quantification of CTX3 C in fish samples.Chapter 3,online detection of aniline and paratoluidine by AuNPs signal amplification with micelle electric capillary chromatography.We propose a detection method which combine the micellar electric capillary chromatography as the first amplification with AuNPs as the secondary online signal amplification,for rapid on-line detection of aniline and paratoluidine.Because of the electrostatic force,the positive charge samples will assemble on the negatively charged micelle surface,forming a congeries with positive charge;then,the negatively charged AuNPs were introduced into the capillary,the AuNPS and micelle will assemble together immediately by the electrostatic force,achieve the goal of enlargeing the detection signal.This method which was used for online detection of aniline and paratoluidine has high sensitivity,simple operation,good stability,etc.The LODs of aniline and paratoluidine achieved 2.656×10-9 mol·L-1 and 0.117×10-9 mol·L-1,respectively.Compared with conventional CZE and pure MEKC,the enrichment ratio was 13421 fold,6740 fold for aniline and 972 fold,361 fold for paratoluidine.