Design And Biological Application Of Amphiphilic And Cell-targetable Fluorescent Probe

Fluorescent dyes as an important branch of chemical science,has a rapid development in recent 10 years.A variety of dyes were developed by researcher,but with the rapid development of the dyes,we can't be only focused on the chemical direction,the application of fluorescent dyes in the field of Life sciences is extremely important in recent years.Because of the rapid development of fluorescence microscopy,the microscopic imaging technology has become a basic means of studying fluorescent dyes.The sub-organelle-targeted fluorescent probe has become a hotspot for researchers because of its excellent specificity,but the fluorescent probes have both good targeting and function have been scarcely developed.In this paper,two kinds of fluorescent probes were designed and developed for the detection of cell membrane fluidity and lysosome.In the second chapter,a near-infrared functional fluorescent probe Mem-Cy3 was designed and synthesized,it was designed to develop the cell membrane fluidity.It is well known that cell membranes have an irreplaceable and important role in cells,the cell membrane controls the entry and exit of the substance and protects the cell.The cell membrane fluidity is the most intuitive parameter for responding to cell membranes,and changes in cell status can cause changes in cell membrane fluidity.The changes of cell status can influence the cell membrane fluidity.The most widely used method for the measurement of cell membrane fluidity is FRAP(Fluorescence Recovery After Photobleaching),because that the cyanine dyes contained the flexible double bond what makes it easy to be light bleached,and greatly reduces the laser intensity used in FRAP experiments,increases the wavelength of the laser and makes the laser damage to the cells in the test has been effectively controlled.The phototoxicity has been reduced greatly.The cyanine dyes are modified to have amphiphilic properties similar to the cell membrane,and the Mem-Cy3 has been obtained.The changes of cell membrane fluidity in defferent states and environments are obtained by FRAP experiments.In the third chapter,we designed and synthesized three fluorescent dyes with rhodamine as the maternal.The SA-SNARF can be used as a fluorescent probe capable of stably tracking lysosomes.Lysosomes are the ultimate place to metabolize cells and contains a variety of hydrolases.The pH of the lysosome is maintained at about 5.Fluorescence probes located in lysosomes are widely studied,but probes that can stable stain in lysosomes are not affected by lysosomal pH changes are relatively few.The increasing of lysosomal pH by stimulating with chloroquine shows that SA-SNARF can stably trace lysosomes.The staining of foam cells showed that SA-SNARF could study the pathology of foam cells at the level of subcellular organelles.