Analysis Of Chemical Constituents And Whitening And Moisturizing Skin Care Product Development Of Chia Seeds

Chia seed as the seeds of Salvia Hispania L.,is one of the most important crops of the former Columbus American civilization,are also FDA-certified safe food.In recent years,chia seed plays the outstanding roles in anti-oxidation,lowering blood pressure,improving cardiovascular and other aspects,attracting more and more domestic and foreign scholars in these areas.In 2014,the People's Republic of China National Health and Planning Commission issued No.10 on the approval of Taco sugar and other five kinds of new food raw materials in the announcement,the chia seed as a new type of food raw materials into the people's attention.In this paper,the chemical composition of the chia seed was analyzed and then developed into new skin care products.The main content and results of the experiment are as follows:1.Analysis of chemical composition of chia seed:The types and content of the fat-soluble components in the seeds were analyzed by GC-MS;The content of amino acids in the seeds was analyzed by RP-HPLC.The content of phenols in the seeds was determined by HPLC.The content of mineral elements in the seeds was determined by Absorption Spectrophotometry.The results showed that 14 kinds of components were isolated from the fat-soluble of chia seed,the content was 0.117 % 9-hexadecenoic acid,8.521 % palmitic acid,0.259 % 14-methylhexadecanoic acid methyl ester,0.094 % 15-methylhexadecanoic acid methyl ester,83.410 % linolenic acid,1.393 % 10-octadecenoic acid,4.233 % stearic acid,0.202 % linoleic acid,0.187 % cis-10-nacitric acid 0.274 % naphthenic acid,0.314 % tridecenoic acid methyl ester,0.415 % arachidic acid,0.258 % behenic acid and 0.324 % tetracosanoic acid.There were also 17 kinds of free amino acids in the seeds,which contain 7 kinds of essential amino acids.The total content was 548.27 ?g/g.There were 19 kinds of total amino acids and 8 kinds of essential amino acids,the total content was 25903.81 ?g/g.The content of phenolic compounds in the seeds was as follows: catechin 81.95 ?g/g,epicatechin 165.73 ?g/g,rutin 61.58 ?g/g,phlorizin 19.2 ?g/g,myricetin148.45 ?g/g,quercetin 120.05 ?g/g.The content of manganese in the mineral was 141.22 mg/kg,sodium was 103.52 mg/kg,magnesium was 5715.13 mg/kg,calcium was 8598.81 mg/kg,iron was113.92 mg/kg,potassium was 13358.62 mg/kg,zinc was 126.13 mg/kg and copper was 38.58 mg/kg.2.Extraction process of chia seed:The antioxidant and whitening abilities were used as indicators,and the single factor was used to determine the extraction concentration of chia seed using 75 % ethanol.The concentration,time,extraction times and liquid to solid ratio were used as orthogonal factors.The DPPH antioxidant capacity was used as indicator for L₉?3₄?orthogonal test.The results showed that ethanol concentration> extraction times> solid-liquid ratio> extraction time.The optimum extraction process was ethanol concentration 65 %,extraction 1 time,the amount of ethanol 12 times,each extraction 2h.3.Prescription process of skin care products of chia seed:The four-factor and three-level orthogonal test was used to analyze the total score of cleansing soap,skin care water,cream and mask of chia seed.The results showed that: 3% amino acid foaming agent,4% chia seed oil,3 % tea tree oil,8 % glycerol,72 % soap base.The prescription of skin care water of chia seed was: 0.2 % chia seed gel,1.5 % chia seed extract,12 % glycerol,1.5 %hydrogenated castor oil,appropriate amount of allantoin,D-panthenol,octanoyl glycine and triethanolamine,100 % water.The prescription of cream was: 1 % xanthan gum,0.2 % chia seed gel,10 % chia seed skin water,8 % Glycerol,0.1 % sodium hyaluronate,appropriate amount of triethanolamine,allantoin,squalane and methylparaben,water 100 %.The prescription of mask of chia seed was: 0.2 % carbomer,0.4 % chia seed gel,10 % glycerol,10 % chia seed skin care water,0.2 % sodium hyaluronate,appropriate amount of allantoin,methylparaben and betaine,water 100 %.4.Quality standards of skin care products of chia seed:The quality standards of skin care products of chia seed were evaluated in this chapter,including efficacy,safety,stability,toxic substance testing and microbiological testing.The efficacy of cleansing soap of chia seed was evaluated by bacteriostatic test and skin care water,cream and mask of chia seed were evaluated by moisturizing and whitening evaluation.The safety of skin care products of chia seed were evaluated by irritation and acute toxicological tests;The stability test of the skin care products were carried out by room temperature retention,centrifugal test and cold tolerance test.Then the arsenic,lead,mercury and microbes were detected as well.The results showed that the soap of chia seed on Aspergillus niger,Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa had a better antibacterial effect.Shu-fu-jia soap on Aspergillus niger,Escherichia coli,golden grapes Cocci had a better bacteriostatic effect,whereas on Pseudomonas aeruginosa did not have antibacterial effect.While Li-shi soap on the four bacteria did not have the antibacterial effect.Whitening efficacy:Skin care water group compared with the blank group and stroma group,the administration group and positive group significantly decreased the melanin content in guinea pigs?P <0.01?.Cream group compared with the blank group,cream group significantly decreased the content of melanin in guinea pigs?p <0.05?.Mask group compared with the blank group and stroma group,administration group?P<0.05?and positive group?P <0.01?significantly decreased the melanin content in guinea pigs.Moisturizing efficacy: Skin care water group compared with the blank group,stroma group and administration group and positive group significantly higher the content of skin water?P <0.01?.Skin care water group compared with the stroma group,administration group significantly higher the content of skin water?P <0.01?.Cream group compared with the blank group,stroma group and administration group,positive group significantly higher the content of skin water?P <0.01?.Cream group compared with the stroma group,administration group significantly higher the content of skin water?P <0.01?.Mask group compared with the blank group,stroma group and administration group,positive group significantly higher the content of skin water?P <0.01?.Mask group compared with the stroma group,administration group significantly higher the content of skin water?P <0.05?.Skin care products of chia seed have better safety and stability,the content of toxic substance and microbial detection are in line with standards.