Studies On The Structure Of Fucoidan From Kjellmaniella Crassifolia And Antitumor Activity Of The Degradation Products

This paper tries to hydrolyze fucoidan by HCl,and showing its effects of antitumor and immunomodulatory.1.Fucoidan F2 was degraded by 0.1 mol/ml hydrochloric acid,and abtained F2-a,F2-b and F2-c.Chemical composition analysis indicated that F2-a?F2-b?F2-c contained 88%?26.2%?54% total polysaccharides,with 6.55%?56.68%?1.87% SO42-;HPLC analysis indicated that F2-a were mainly composed of mannose and glucuronic acid,F2-b were composed of mannose?glucuronic acid and fucose,F2-c was just fucose;The results of IR spectrum indicated that absorption peaks of O-H(3369-3403cm-1)in the F2-a and F2-b,;S=O(1144cm-1)existed F2-b,which demonstrated that F2-b contain sulfate substitute.13 C NMR spectrum indicated thatthe displacement of C2-C5 was 62-75 ppm of F2-a?F2-b and F2-c,and 18.13-18.73 ppm is the displacement of C6 of F2-b and F2-c.2.The result for inhibiting HepG2 ? MCF-7 ? Hela ? A549 ? HT29 and RAW264.7 growth in vitro indicated that inhibition ratio of F2-b on HepG2 was showed 36%,F2-c showed with inhibition ratio 32% on Hela,the inhibition ratio of F-2 and F2-c on RAW264.7were 43.26%?42.12%,respectively at 800 ?g/ml;no effect on MCF7 in all samples.3.The results of MTT and Elisa Assay showed that effect of F2?F2-a?F2-b and F2-c on RAW264.7.The contents of secretion of cytokines TNF-? ? GM-CSF of F2 ? F2-a were 27.131 ng / L and 286.96 ng / L,respectively at the concentration of 20?g / mL.