Preparation And Characteristics Of PLA Multi-dimensional Scaffold Materials And Its Use In Tissue Engineering

Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering.Emulsion electrospinning is a novel method to produce shaped nanofibers composed of a mixture of two or more components,which is very convenient and suitable for the tissue engineering scaffolds.Therefore,in our study,we used polylatic acid(PLA)solution as continuous phase,and chose denionized water and Sodium alginate(SA)as dispersion phase respectively,preparing W/O emulsions and then electrospinning to get two-dimensional and threedimensional nanofiber scaffolds by changing collecting substrates.We fabricated SA/PLA composite nanofiber scaffolds with a single setup by emulsion electrospinning.The possibility of having SA/PLA nanofibers scaffolds had been confirmed by SEM images,and CLSM images and FT-IR spectra proved that SA was located on the surface of composite fibers.Moreover,calcium ion replacement step was used to anchor the alginic ion in a form of gelated calcium alginate(CA)and the composite scaffolds are beneficial for cell proliferation and differentiation.In order to fabricate porous nanofiber scaffolds,H2O/PLA emulsions were used to electrospinning firstly.By changing the solid collected substrate into dynamic liquid collected substrate and removing the solvent by vacuum freeze-drying,we got the PLA three-dimensional porous nanofiber scaffolds.Moreover,we used SA solution as dispersion phase and selected CaCl2 as liquid collected substrate in order to keep the alginic ion,getting the CA/PLA composite three-dimensional nanofiber scaffolds.Overall,emulsion electrospinning is an innovated way to fabricate composite nanofiber scaffolds,and the two-dimensional as well as three-dimensional scaffolds fabricated by this method will have a potential application in tissue engineering.