Experimental Study Of PM2.5 On Acute Oxidative Damage Of Ovary In SD Rats

Objective: through the methods of exposure to PM_2.5 by intratracheal instillation of different concentrations,observe the degree of oxidative stress in blood,lung,ovary,changes of oxidative damage and antioxidant substances,lung,ovarian blood,acute oxidative damage particles on ovary.Methods: female SD rats were randomly divided into control group?saline?and low,middle and high?3.75,7.5,3 15mg/kg?3 dose group,12 rats in each group,using intratracheal exposure method and exposure,disposable tracheal injection,infusion volume was 3mL/ kg respectively,perfusion with different concentrations of particles in suspension,control group with normal saline perfusion volume?the injected solution,temperature of 37 degrees celsius?,exposure after fasting water after intraperitoneal injection of 24 h anesthesia,the left ventricle bleeding rats were killed and blood samples,determination of superoxide dismutase?SOD?activity and malondialdehyde?MDA?content.The SOD,MDA and protein carbonyl in lung tissue and ovary were detected after removal of lung and ovarian tissue.Results: compared with the control group,the rats exposed to blood SOD activity decreased,middle and high dose groups,the difference has statistical significance?P<0.05?.The comparison between the high dose group and low dose group,with significant difference?P<0.05?.MDA content in the blood were increased,the difference was statistically significant?P<0.05?,and the content of high dose group was much higher than that of other groups,the difference was statistically significant;Compared with the control group,the exposure groups.Rat lung tissue SOD activity decreased,the middle and high dose groups,the difference has statistical significance?P<0.05?;between the middle and high dose group and low dose group compared to the same with statistical significance?P<0.05?.Compared with the control group,the content of MDA in the lung tissue of each group was increased,and the difference between the middle and high dose groups was also statistically significant?P<0.05?.Compared with the control group,each exposure group,the rats lung tissue of protein carbonyl content increased,the difference between the high dose group has significant difference?P< 0.05?and the content in high dose group were much higher than the other groups,the difference is statistically significant?P <0.05?;Compared with the control group,high dose exposure group rat ovarian tissue SOD activity decreased and the differences have statistical significance?P<0.05?;between the middle and high dose group and low dose group compared with statistical significance?P<0.05?.Compared with the control group,the levels of MDA in the ovarian tissue of all rats were increased,but only the high dose group was statistically significant?P<0.05?.Compared with the control group,the levels of protein carbonyl in the ovarian tissue of all rats were increased,but the difference was not statistically significant?P>0.05?;Conclusion: PM_2.5 can cause female SD rats in vivo oxidation and antioxidant system imbalance,resulting in oxidative stress response,PM_2.5 on ovarian exist acute oxidative damage,but not as good as of lung impact.It was also found that the lipid membrane proteins were on oxidative stress are more sensitive.