Determination Of Uranium And ATP Based On Bipolar Bidentate Complex Emission Spectroscopy

ATP is a very important coenzyme drugs and high energy compound.It can improve the metabolism of the body fat,protein,nucleic acid.ATP can not only treat the diseases caused by cell damage in the body,but also can assist in the treatment of heart failure,myocarditis,myocardial infarction,cerebral arteriosclerosis and other diseases.Therefore,the quality monitoring of ATP for the study of cells and the body's metabolic processes and physiological activity,drug sensitivity testing and food safety control are of great significance.Uranium is a rare actinide transition metal and uranium-235 is a very important fuel for nuclear power generation.But uranium and its compounds are radioactive and toxic,posing a threat to the environment and people's health.To efficiently obtain uranium resources and prevent uranium pollution,the establishment of fast and sensitive and selective detection of uranium method is of great significance.Therefore,it is important to monitor the quality of ATP drugs and heavy metals uranium in drugs or in the environment.In this paper,a bipolar bidentate ligand?Isophthalaldehyde-tetrapyrrole,IPTP?was synthesized and its structure and spectral properties were investigated.A new resonance light scattering?RLS?method for the determination of uranium using this ligand as a probe and the second-order scattering?SOS?analysis based on IPTP-uranyl complexes were proposed.These methods provide a new means for the determination of uranium and ATP.The first part introduces the characteristics of detection methods of uranium and ATP and the current research progress.The properties and applications of IPTP and its metal complexes,the progress of metal polymer research and the application of resonance light scattering and second-order scattering were introduced.In the second chapter,we synthesized the bipolar bidentate ligand IPTP which was characterized.The ligand could be self-assembled with uranyl ions to form dimer and supramolecular polymer,which greatly enhanced the resonant optical signal of the system.Based on this,we established a method for the detection of uranium in the solution using the ligand as a probe.The detection limit of uranium?VI?was 0.003 nmol mL^-1 under the most suitable conditions.The method has been used in the actual sample of uranium?VI?detection with the recovery rate between 98.0¹01.0%.In the third chapter,we study the spectral characteristics of BUIPTP-ATP system by the dinuclear uranyl complexes of the coordination of bipolar bidentate ligands and uranyl.A new method for the direct determination of ATP in solution without separation by second-order scattering method with high sensitivity was established.We also studied the influencing factors and the optimum reaction conditions.The linear range of second-order scattering intensity and ATP concentration is 2.5⁵00 nmol L^-1 with the detection limit of 0.75 nmol L^-1 under suitable conditions.The method was successfully used for the determination of ATP in the sample.