| Silk is a natural fiber,mainly composed of silk fibroin and sericin.Silk fibroin accounted for about 70% ratio,and the silk fibroin is relatively easy to extract.Silk fibroin contains up to 18 different types of amino acids,of which 8 kinds of amino acids are essential for human body.In addition,silk fibroin has good biocompatibility and is widely used in biomedicine.Studies have shown that silk fibroin has a hemostatic effect,but compared with other hemostatic materials such as chitosan and porous zeolite,the hemostatic time is too long and the hemostatic effect needs to be improved.Gelatin is also a natural polymer compound that contains 18 amino acids of protein,mainly from the animal's collagen partial hydrolysis.Gelatin was discovered by people earlier with hemostatic materials,gelatin sponge has been widely used in clinical.In this paper,silk fibroin as the main ingredient,adding the right amount of gelatin,the silk fibroin protein and gelatin were blended to prepare soluble fibroin / gelatin composite hemostatic material and insoluble fibroin / gelatin composite hemostatic material(addingglycerin group).The microstructures of the two materials were investigated by scanning electron microscopy(FESEM),Fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD).The coagulation index(BCI),clotting time in vitro(APTT),prothrombin time(PT),hemorheology,platelet adhesion rate,platelet activating factor(PF4),rat tailing test and rat liver hemostasis test were performed on fibroin / gelatin composite Hemostatic performance of the inquiry,the main conclusions are as follows:1.Hydrolyzed dialysis of regenerated silk fibroin and gelatin solution by a certain percentage of blending,freeze-dried to obtain a soluble fibroin / gelatin composite material.The results of FESEM,FTIR and XRD indicated that the soluble fibroin / gelatin composites had a porous structure;fibroin and gelatin were physically blended;and the silk fibroin secondary structure was still Silk I.The results of the hemostatic tests showed that compared with the pure silk fibroin materials,the hemostatic indexes of the composite materials increased and the hemostatic effect was enhanced.The group with 7: 3 fibroin and gelatin ratio had The best hemostatic effect.Such as coagulation index was 34.30 ± 0.96%,in vitro clotting time was 5.70 ± 0.31 min,tail stop bleeding time and liver hemostasis time were 5.50 ± 0.46 min and 2.56 ± 0.18 min and so on.However,during the experiment,it was found that most of the dissolution of the composite material in contact with blood may affect the hemostatic performance.It is therefore desirable to modify the composite to a poorly soluble hemostatic material by adding suitable reagents.2.Hydrolyzed dialysis fibroin and gelatin blend,while adding a small amount of glycerol as a plasticizer,freeze-dried to obtain insoluble composite hemostatic material.FESEM,FTIR and XRD were used to characterize the materials.The results showed that there was still porous structure inside the material compared with the non-glycerin composites;the chemical properties of silk fibroin and gelatin did not change;I transition to Silk II.Hemostatic properties of insoluble fibroin / gelatin composites were characterized by water absorption and various hemostatic properties.The results showed that the hemostatic effect of the insoluble fibroin / gelatin composite was better than that of the soluble group.Among them,the fibroin and gelatin ratio of 8: 2 group of poorly soluble composite material has the best hemostatic effect.Such as coagulation index was 16.40 ± 0.64%,in vitro clotting time was 2.87 ± 0.12 min,tail stop bleeding time and liver bleeding time were 3.27 ± 0.31 min and 1.88 ± 0.03 min and so on. |
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